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Sour milk containing phosphatidyl choline and preparation method thereof

A technology for fatty acylcholine and yogurt, which is applied in the field of phosphatidylcholine-containing yogurt and its preparation, and can solve problems such as narrow audience range, high purity requirements, and cumbersome extraction methods

Inactive Publication Date: 2017-11-10
WUHU FOMAN BIOPHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the above-mentioned prior art, the purpose of the present invention is to overcome the fact that phosphatidylcholine often appears as a health product in the prior art, so that its audience is relatively narrow, and it is often extracted due to its high purity requirements, resulting in the extraction of phosphatidylcholine The method is cumbersome, thereby providing a yoghurt containing phosphatidylcholine and a preparation method thereof that has a good mouthfeel and is suitable for daily consumption by most people, especially for children

Method used

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Examples

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Effect test

preparation example Construction

[0014] The invention provides a method for preparing phosphatidylcholine-containing yoghurt, wherein the preparation method comprises:

[0015] 1) mixing bovine pancreas with ethanol and extracting, filtering off the filter residue to obtain extract M1;

[0016] 2) Add acetone to the extract M1 prepared in step 1), shake it, then let it stand, remove the acetone layer, and prepare the mixture M2;

[0017] 3) eluting the mixture M2 prepared in step 2) sequentially through an ion exchange resin and a silica gel column to obtain a primary product M3 containing phosphatidylserine;

[0018] 4) The initial product M3 is mixed with milk and then fermented to prepare phosphatidylcholine-containing yoghurt.

[0019] In the present invention, bovine pancreas is mixed with ethanol and extracted, and the extract is taken, then acetone is added to the extract for vibration and left to stand, the acetone layer is removed, and the ion exchange resin and silica gel column are further eluted,...

Embodiment 1

[0030] 1) Mix 10 parts by weight of bovine pancreas with 150 parts by weight of ethanol, place it at a temperature of 30° C. for 5 hours, filter off the filter residue, and obtain the extract M1;

[0031] 2) Add 250 parts by weight of acetone to the extract M1 prepared in step 1), then ultrasonically vibrate for 5 minutes, then let it stand for 20 minutes, remove the acetone layer, and prepare the mixture M2;

[0032] 3) The mixture M2 prepared in step 2) is eluted with an ion exchange resin for 1 min and a silica gel column for 3 min to obtain the primary product M3 containing phosphatidylserine;

[0033] 4) The initial product M3 and 100 parts by weight of milk were mixed and then fermented at 35°C for 8 hours to prepare phosphatidylcholine-containing yoghurt A1. (phosphatidylcholine content is 3.12%)

Embodiment 2

[0035] 1) Mix 10 parts by weight of bovine pancreas with 250 parts by weight of ethanol, place it at a temperature of 40° C. for 10 hours, filter off the filter residue, and obtain the extract M1;

[0036] 2) Add 350 parts by weight of acetone to the extract M1 prepared in step 1), then ultrasonically vibrate for 15 minutes, then let it stand for 60 minutes, remove the acetone layer, and prepare the mixture M2;

[0037] 3) The mixture M2 prepared in step 2) was eluted with an ion exchange resin for 5 minutes and a silica gel column for 10 minutes to obtain the primary product M3 containing phosphatidylserine;

[0038] 4) The initial product M3 and 120 parts by weight of milk were mixed and then fermented at 40° C. for 15 hours to prepare phosphatidylcholine-containing yoghurt A2. (Phosphatidylcholine content is 2.56%)

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Abstract

The invention discloses sour milk containing phosphatidyl choline and a preparation method thereof. The preparation method of the sour milk containing the phosphatidyl choline comprises the following steps: 1), mixing bovine pancreas with ethanol, carrying out extraction on the mixture, and carrying out filtering so as to remove residues to obtain an extract M1; 2), adding acetone into the extract M1 prepared in the step 1), carrying out vibration, carrying out standing, and removing an acetone layer so as to obtain a mixture M2; 3), sequentially passing the mixture M2 prepared in the step 2) through ion exchange resin and a silica gel column so as to carry out elution to obtain a preliminary product M3 containing phosphatidyl choline; and 4), mixing the preliminary product M3 with milk, and fermenting the mixture so as to prepare the sour milk containing the phosphatidyl choline. By adopting the design, a relatively good taste is realized; and thus, the prepared sour milk containing the phosphatidyl choline is suitable for daily eating of the most people. The sour milk containing the phosphatidyl choline is especially suitable for daily eating of children.

Description

technical field [0001] The invention relates to the field of production and preparation of food containing phosphatidylcholine, in particular to yoghurt containing phosphatidylcholine and a preparation method thereof. Background technique [0002] Phosphatidylcholine is widely used in food because of its unique physical and chemical properties and physiological properties. However, in daily life, it often appears as a health product, which makes its audience narrow. At the same time, phosphatidylcholine has a good health care effect on the human brain, especially on the brain development of children. [0003] Therefore, to provide a phosphatidylcholine-containing yoghurt and a preparation method thereof that has a good taste and is suitable for daily consumption by most people, especially for children, is an urgent problem to be solved by the present invention. Contents of the invention [0004] In view of the above-mentioned prior art, the purpose of the present inventi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23C9/13
CPCA23C9/13
Inventor 张慈铭
Owner WUHU FOMAN BIOPHARMA CO LTD
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