Multichannel microfluidic chip device capable of detecting plurality of subtype swine influenza at same time

Abstract

The invention relates to a multichannel microfluidic chip device capable of detecting a plurality of subtype swine influenza at the same time, and belongs to the field of analytical tests. A plurality of obstacles exist in using cheap and easily processed polydimethylsiloxane(PDMS) to prepare a substrate of a microfluidic chip used for diagnosing subtype swine influenza; the PDMS has a spontaneous slow deformation property, which causes gradual narrowing of a chip pipeline; a PDMS surface is strongly hydrophobic, so that surface chemical modification effects are difficult to sustain; and the surface adsorbs and swallows biomacromolecules. A key point of the scheme is that a substrate material is PDMS with an original ecological surface and the chip pipeline is filled with micron-sized silica particles, and the functions of the micron-sized silica particle fillers comprise supporting pipeline inner walls, substituting and compensating original hydrophobic pipelines by a hydrophilic microfluidic channel collection, and reducing the contact chances between the biomacromolecules and inner wall faces by rigidly squeezing and covering up the inner wall faces.

Description

technical field [0001] The invention relates to a multi-channel microfluidic chip device for simultaneously detecting multiple subtypes of swine influenza, belonging to the field of analysis and testing. Background technique [0002] For the technical background of multi-channel microfluidic subtype swine influenza diagnosis, please refer to CN 201110311127.1 and other invention patent applications. [0003] As far as the overall overview of microfluidic technology itself is concerned, you can refer to the monograph "Illustrated Microfluidic Chip Laboratory" published by the famous microfluidic expert Mr. Lin Bingcheng not long ago, which has been published by Science Press. The past, present, and future prospects of microfluidic technology, etc., have detailed and long-form discussions that go deep into specific details. [0004] So, let's talk about the key issues of this case. [0005] The basic structure of a microfluidic chip includes a substrate etched with channels ...

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Problems solved by technology

[0007] But it's not that simple

[0008] First, this polydimethylsiloxane material, which is referred to by the acronym PDMS, is itself a strongly hydrophobic material, and channels are built on this material. modification operation, then, after the overall assembly is completed, that is, after the cover is covered, because the inner surface of the channel in the structure occupies most of the inner surface of the liquid flow channel, then the inner surface of the PDMS channel is The strong hydrophobicity is the decisive factor, it will make it very difficult for the polar liquid fine flow similar to the aqueous solution to pass through, and its flow resistance is so large that even the general micropump is difficult to push, of course, if the cover sheet is also If you choose to use the PDMS material, then the problems are basically the same, with minor differences; therefore, in the prior art, it is necessary to modify and modify the inner surface of the channel on the PDMS material; then, this is for the PDMS channel Is the modification operation of the inner surface of the channel very troublesome? That's not the problem. What constitutes a serious technical problem is another problem: the PDMS polymer molecules in the bulk phase of the PDMS material substrate have the characteristics of automatic diffusion and migration to the surface. The characteristics of polymer molecules diffusing and migrating to the surface automatically will make the modified state of the inner surface of the channel modified by the surface unable to maintain for a long enough time. The maintenance time of the internal surface state is roughly only enough to complete the time required for the internal test experiment in the laboratory; in other words, the surface state of the PDMS channel inner surface after surface modification or surface modification is formed after modification It cannot last for a long time, but it will automatically tend to or change back to the surface state before the surface modification, and return to the original strongly hydrophobic surface state in a short period of time. Then, just imagine, such a slight Can flow control chips be produced in large quantities, stored in large quantities, and widely promoted? The answer is obvious, that is, impossible

This problem has also existed for many years, and so far, it has not been properly solved

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