A formula and method for cryopreservation and hatching of artemia shelled eggs
A technology for cryopreservation and artemia eggs, which is applied in the preservation of human or animal bodies, adaptation to climate change, fish farming, etc. It can solve the problems of low hatching rate and achieve high hatching rate, easy-to-obtain formula and simple method Effect
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Embodiment 1
[0033] (1) Shelling process:
[0034] (1-1) Hydrate the eggs of Artemia for 1 hour, use salt water with a salinity of 1% for hydration, and fill in air during the hydration process to prevent the eggs from sinking to the bottom, which is not conducive to hydration.
[0035] (1-2) After fully absorbing water, add the shelling liquid according to the ratio of adding 600mL shelling liquid per 100g of Artemia eggs, stir and inflate continuously, and the gas volume is sufficient to ensure uniform inflation; A lot of heat will be generated during the shelling process, and the temperature of the shelling liquid will rise. Therefore, the shelling process requires an ice bath and constant stirring and aeration, and the temperature is controlled at 4°C. The color of Artemia eggs changes from brown to off-white, and finally orange-red, indicating shelling. Completely, the shelling time is 15min.
[0036] (1-3) After the shelling is completed, collect the shelled eggs with a 200-mesh scr...
Embodiment 2
[0044] (1) Shelling process:
[0045] (1-1) Hydrate the eggs of Artemia for 1 hour, use salt water with a salinity of 1% for hydration, and fill in air during the hydration process to prevent the eggs from sinking to the bottom, which is not conducive to hydration.
[0046] (1-2) After fully absorbing the water, add the shelling liquid according to the ratio of adding 600mL shelling liquid per 100g of Artemia eggs, and add the shelling liquid to continuously stir and inflate. The gas volume is sufficient to ensure uniform inflation; During the shelling process, a lot of heat will be generated, and the temperature of the shelling liquid will rise. Therefore, the shelling process requires an ice bath and constant stirring and aeration, and the temperature is controlled at 4°C. The shell is complete, and the shelling time is 15 minutes.
[0047] (1-3) After the shelling is completed, collect the shelled eggs with a 200-mesh screen, rinse with a large amount of fresh water, and r...
Embodiment 3
[0055] (1) Shelling process:
[0056] (1-1) Hydrate the eggs of Artemia for 1 hour, use salt water with a salinity of 1% for hydration, and fill in air during the hydration process to prevent the eggs from sinking to the bottom, which is not conducive to hydration.
[0057](1-2) After fully absorbing the water, add the shelling liquid according to the ratio of adding 600mL shelling liquid per 100g of Artemia eggs, and add the shelling liquid to continuously stir and inflate. The gas volume is sufficient to ensure uniform inflation; During the shelling process, a lot of heat will be generated, and the temperature of the shelling liquid will rise. Therefore, the shelling process requires an ice bath and constant stirring and aeration, and the temperature is controlled at 4°C. The shell is complete, and the shelling time is 15 minutes.
[0058] (1-3) After the shelling is completed, collect the shelled eggs with a 200-mesh screen, rinse with a large amount of fresh water, and ri...
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