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Preparation method of high-performance rhamnolipid emulsified asphalt

A technology of rhamnolipid and emulsified asphalt, applied in biochemical equipment and methods, microorganism-based methods, microorganisms, etc., to achieve good fluid loss reduction and inhibition, strong surface activity and emulsifying ability, and good emulsifying effect. Effect

Inactive Publication Date: 2017-11-17
南京东威万合新材料科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are few studies on the application of rhamnolipids as emulsifiers in emulsified asphalt

Method used

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  • Preparation method of high-performance rhamnolipid emulsified asphalt

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Embodiment 1

[0022] A preparation method of high-performance rhamnolipid emulsified asphalt, comprising the following steps:

[0023] Step 1: Pseudomonas aeruginosa was inoculated on the slant medium, and cultured at a constant temperature of 37° C. for 24 hours;

[0024] Step 2: Pick the colony on the slant medium and connect it to the seed medium, the liquid volume is 50mL / 250mL, the temperature is 37°C, and the shaker speed is 200r min -1 , cultivated at constant temperature for 24 hours to obtain the seed solution;

[0025] Step 3: Receive the seed liquid into the fermentation medium with an inoculum amount of 8% (v / v), the liquid volume is 50mL / 250mL, the temperature is 37°C, and the shaker speed is 200r·min -1 , cultivated at a constant temperature for 7 days to obtain a fermentation broth;

[0026] Step 4: Centrifuge the fermentation broth at 8000r / min for 15min, take the supernatant, adjust the pH to 2, add 3 times the volume of ethyl acetate for extraction, collect the organic p...

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Abstract

The invention discloses a preparation method of high-performance rhamnolipid emulsified asphalt, and belongs to the technical field of asphalt preparation. The method comprises the following steps: inoculating an inclined medium with Pseudomonas aeruginosa, inoculating a seed medium with a bacterial colony on the inclined medium, inoculating a fermenting medium with the above obtained seed liquid, extracting rhamnolipid through ethyl acetate, and preparing the emulsified asphalt. The rhamnolipid has high surface activity and emulsifying ability, can be completely biodegraded, and is friendly to the environment. The prepared high-performance rhamnolipid emulsified asphalt has the advantages of good emulsification effect, good stability, good filtrate reduction property and good inhibition property.

Description

technical field [0001] The invention belongs to the technical field of asphalt preparation, and in particular relates to a preparation method of high-performance rhamnolipid emulsified asphalt. Background technique [0002] Emulsified asphalt is a uniform and stable emulsion formed by heating viscous asphalt to a fluid state, and then forming micro-droplets dispersed in water with emulsifier-stabilizer through the action of mechanical force. Emulsified asphalt is a thermodynamically unstable system. Its stability is caused by the various effects of emulsifiers and emulsion stabilizers added from the outside. The current emulsified asphalt has the problem of insufficient stability. [0003] Rhamnolipid is a kind of glycolipid biosurfactant, which has strong surface activity and emulsifying ability, can be completely biodegraded, and is friendly to the environment. potential application value. However, there are few studies on the application of rhamnolipids as emulsifiers ...

Claims

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Application Information

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IPC IPC(8): C08L45/00C08L53/02C08L91/00C08K5/12C08K5/1545C08J3/11C12P19/44C12R1/385
CPCC08L45/00C08J3/11C08J2345/00C08J2453/02C08J2491/00C08L2205/03C12P19/44C08L53/02C08L91/00C08K5/12C08K5/1545
Inventor 章云徐东章玮
Owner 南京东威万合新材料科技有限公司