Application of alpha-silver vanadate as mimic enzyme
A technology of silver vanadate and imitating enzymes, which is applied in the field of imitating enzymes, can solve the problems of undeveloped application potential and achieve good HRP enzyme catalytic activity and potential application prospects
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Embodiment 1
[0019] Preparation process of silver vanadate:
[0020] Will NH 4 VO 3 Configured into an aqueous solution with a concentration of 0.029mol / L, and then AgNO 3 Configured into an aqueous solution with a concentration of 0.029mol / L, AgNO 3 Solution and NH 4 VO 3 The solution is directly blended, and the above mixed solution is crystallized at 30°C for 1 hour. After crystallization, it is washed with deionized water and ethanol, filtered, and the filter cake is dried at 60°C for 24 hours to obtain a chemical composition of AgVO 3 Of alpha-vanadate ( figure 1 with 2 ).
[0021] by figure 1 with 2 It can be seen that the synthesized product is micron-sized rod-shaped silver α-vanadate with a length of 2-10 microns and a width of about 500 nanometers.
[0022] Utilize typical catalytic oxidation substrates TMB and H 2 O 2 The peroxidase-like activity of the synthesized silver vanadate was measured. Take a 1.5mL centrifuge tube, add 800μL phosphate buffer (100mM, pH 4.0), and then add 1mM T...
Embodiment 2-8
[0024] Preparation Process:
[0025] Will NH 4 VO 3 Configured into an aqueous solution with a concentration of 0.005~0.050mol / L, and then AgNO 3 Configured into an aqueous solution with a concentration of 0.005~0.050mol / L, AgNO 3 Solution and NH 4 VO 3 The solution is directly blended, and the above mixed solution is crystallized at 10-50℃ for 0.5-2 hours, washed after crystallization, filtered, and the filter cake is dried at 40-60℃ for 24 hours to obtain a chemical composition of AgVO 3 Of α-Vanadate (see Table 1).
[0026] Take a 1.5mL centrifuge tube, add 800μL phosphate buffer (100mM, pH 4.0), and then add 1mMTMB and 0.5mM H 2 O 2 As a catalytic substrate, the 20μg / mL nanometer mimetic enzyme materials synthesized in Table 1 were finally added. When the nanometer mimetic enzyme material is added to the reaction system, the rapid color change (from colorless to blue) can be observed with the naked eye. The absorbance of all reaction systems was measured by U-2900 Hitachi ultra...
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