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A kind of application of silver vanadate as mimetic enzyme

A technology of silver vanadate and imitating enzymes, which is applied in the field of imitating enzymes, can solve the problems of undeveloped application potential and achieve good HRP enzyme catalytic activity and potential application prospects

Active Publication Date: 2017-01-11
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Silver vanadate has a unique crystal structure, and its application potential in the biological field has not yet been developed

Method used

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  • A kind of application of silver vanadate as mimetic enzyme
  • A kind of application of silver vanadate as mimetic enzyme
  • A kind of application of silver vanadate as mimetic enzyme

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Silver vanadate preparation process:

[0020] Will NH 4 VO 3 Configured as an aqueous solution with a concentration of 0.033mol / L, and then AgNO 3 Configured as an aqueous solution with a concentration of 0.067mol / L, AgNO 3 The solution was added dropwise to NH 4 VO 3 solution, then add ammonia water dropwise to adjust the pH of the solution to 7; crystallize the above mixed solution at 160-200°C for 24-48 hours, wash with deionized water and ethanol successively after crystallization, filter, and filter The cake was dried at 40-60°C for 24 hours to obtain the chemical composition AgVO 3 of silver vanadate ( figure 1 and 2 ).

[0021] Depend on figure 1 and 2 It can be seen that the synthesized product is band-shaped silver vanadate with a length of 0.5 to several microns and a width of about 100 nm.

Embodiment 2

[0023] Utilizing typical catalytic oxidation substrates TMB and H 2 o 2 The peroxidase-like activity of the synthesized silver vanadate was determined.

[0024] Silver vanadate simulates the effect of peroxidase on the substrate TMB: take a small centrifuge tube, add 800μL phosphate buffer (100mM, pH 5.0), then add 8mM TMB and 5mM H 2 o 2 As substrate, add 300 μg mL at the end -1 AgVO 3 Nanobelts. When AgVO 3 After adding nanobelts to the reaction system, the color change of the system can be observed with the naked eye. The absorbance of all reaction systems is detected under the condition of wavelength 652nm by Hitachi U-2900 UV spectrophotometer using time scanning mode (see image 3 ).

[0025] Depend on image 3 (A), the color change of the solution of TMB under the catalysis of different systems can be observed, (a) H 2 o 2 +AgVO 3 nanobelts system, the solution is colorless and transparent, (b) TMB+AgVO 3 nanobelts system, the solution is colorless and tran...

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Abstract

The invention relates to a technology of mimic enzymes, in particular to an application of silver vanadate serving as a mimic enzyme. According to the application of silver vanadate serving as the mimic enzyme, silver vanadate is obtained through hydro-thermal synthesis. Silver vanadate has excellent HRP (horseradish peroxidase) catalytic activity, has potential application prospect in the fields such as immunoassay and the like and is cheap and easy to obtain.

Description

technical field [0001] The invention relates to the technology of imitating enzymes, in particular to the application of silver vanadate as an imitating enzyme. Background technique [0002] Peroxidase (Horseradish peroxidase, referred to as HRP) is a kind of oxidoreductase produced by microorganisms or plants, and uses hydrogen peroxide as an electron acceptor to catalyze the oxidation of substrates. HRP is one of the most commonly used tool enzymes in immunoenzyme labeling technology, and is often used to label antibodies. [0003] Literature (Nature Nanotechnology, 2007, 2, 577-583) first reported Fe 3 o 4 Magnetic nanoparticles have HRP catalytic activity, and their catalytic efficiency, mechanism, and substrate specificity are the same as those of HRP. And compared with HRP, it also has the advantages of high stability, simple preparation process and recyclable utilization. in Fe 3 o 4 Afterwards, nanomaterials such as graphene oxide, carbon nanotubes, cerium oxid...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J23/68
CPCB01J23/682B01J2523/18B01J2523/55C01G31/00C01P2002/72C01P2004/03C01P2004/04C01P2004/17
Inventor 相振波张盾
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI