Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of imitation enzyme material and its preparation and application

A technology of simulating enzymes and manganese selenide, applied in the field of simulating enzymes, can solve the problems of unexploited application potential in the biological field, and achieve the effects of good HRP enzyme catalytic activity and potential application prospects.

Inactive Publication Date: 2016-07-13
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Manganese Selenide in Mn 2+ There is a strong sp-d electron exchange interaction between the 3d electronic state and the electron / hole band ground state, and has a unique crystal structure. In addition to the traditional electro-optical magnetic field, its application potential in the biological field has not yet been developed

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of imitation enzyme material and its preparation and application
  • A kind of imitation enzyme material and its preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The chemical composition of the manganese selenide mimic enzyme material is MnSe.

[0017] Preparation Process:

[0018] Dissolve 4 mmol of manganese acetate in 60 mL of deionized water, add 10 mL of glycerol, then add 2 g of sodium dodecylsulfonate, followed by 2 mmol of SeO 2 , finally add 10mL of hydrazine hydrate, stir evenly, transfer to a 100mL hydrothermal kettle for crystallization at 180°C for 36 hours, wash with deionized water and ethanol, filter, and dry the filter cake at 60°C for 24 hours to obtain the chemical composition of MnSe manganese selenide mimic enzyme material. XRD test result shows that product is manganese selenide (see figure 1 ).

[0019] Accurately weigh 0.01g of manganese selenide, add 1mL of ultrapure water, shake evenly, take 200μL and add 1mL of TMB chromogenic agent, after the reaction is complete, it will appear blue, and its absorbance value at 652nm wavelength is 2.56, indicating that the prepared manganese selenide material Has...

Embodiment 2-8

[0021] Preparation Process:

[0022] Will Mn 2+ Dissolve the soluble salt in deionized water to form a solution with a concentration of 0.05-0.08mol / L, then mix the aqueous solution and glycerin at a volume ratio of 6:1, and then add a certain amount of sodium lauryl sulfate to make The concentration of sodium lauryl sulfate is 20-40g / L, then add a certain amount of SeO 2 , so that SeO 2 The concentration is 0.02~0.04mol / L, mixed with SeO 2 The aqueous solution of glycerin and hydrazine hydrate are then configured into a mixed solution in a volume ratio of 7:1. The mixed solution is crystallized at 160-200°C for 24-48 hours, washed with deionized water and ethanol, filtered, and the filter cake is dried at 40-60°C for 24 hours to obtain a manganese selenide mimetic enzyme whose chemical composition is MnSe Materials (see Table 1).

[0023] Accurately weigh 0.01g of manganese selenide, add 1mL of ultrapure water, shake evenly, take 200μL and add 1mL of TMB color developer,...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
absorbanceaaaaaaaaaa
absorbanceaaaaaaaaaa
Login to View More

Abstract

The invention relates to a mimetic enzyme technology, in particular to a manganese selenide mimetic enzyme material and the preparation and application thereof. The manganese selenide mimetic enzyme material is prepared by hydrothermal synthesis. The prepared manganese selenide mimetic enzyme material has the catalytic activity of peroxidase.

Description

technical field [0001] The invention relates to an enzyme-mimicking technology, in particular to an enzyme-mimicking material and its preparation and application. Background technique [0002] Peroxidase (Horseradishperoxidase, referred to as HRP) is a type of oxidoreductase produced by microorganisms or plants, and uses hydrogen peroxide as an electron acceptor to catalyze the oxidation of substrates. HRP is one of the most commonly used tool enzymes in immunoenzyme labeling technology, and is often used to label antibodies. [0003] Fe 3 o 4 Magnetic nanoparticles have HRP catalytic activity, and their catalytic efficiency, mechanism, and substrate specificity are the same as those of HRP. And compared with HRP, it also has the advantages of high stability, simple preparation process and recyclable utilization. in Fe 3 o 4 Afterwards, nanomaterials such as graphene oxide, carbon nanotubes, cerium oxide, and manganese oxide were also reported to have HRP catalytic act...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C01B19/04B01J23/34
CPCB01J27/0573C01B19/007C01P2002/72
Inventor 王毅张盾相振波
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI