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Mimetic enzyme material and preparation and application thereof

A technology of simulating enzymes and manganese selenide, which is applied in the field of simulating enzymes, can solve the problems of undeveloped application potential in the biological field, and achieve good HRP enzyme catalytic activity and potential application prospects

Inactive Publication Date: 2015-03-04
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Manganese Selenide in Mn 2+ There is a strong sp-d electron exchange interaction between the 3d electronic state and the electron / hole band ground state, and has a unique crystal structure. In addition to the traditional electro-optical magnetic field, its application potential in the biological field has not yet been developed

Method used

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  • Mimetic enzyme material and preparation and application thereof
  • Mimetic enzyme material and preparation and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The chemical composition of the manganese selenide mimetic enzyme material is MnSe.

[0017] making process:

[0018] Dissolve 4 mmol of manganese acetate in 60 mL of deionized water, add 10 mL of glycerol, then add 2 g of sodium dodecyl sulfonate followed by 2 mmol of SeO 2 Finally, add 10 mL of hydrazine hydrate, stir evenly and transfer to a 100 mL hydrothermal kettle for crystallization at 180°C for 36 hours, wash with deionized water and ethanol, filter, and dry the filter cake at 60°C for 24 hours to obtain a chemical composition of MnSe manganese selenide-mimicking enzyme material. XRD test results show that the product is manganese selenide (see figure 1 ).

[0019] Accurately weigh 0.01g of manganese selenide, add 1mL of ultrapure water, shake evenly, take 200μL and add 1mL of TMB color developer, after the reaction is complete, it will turn blue, and its absorbance value at 652nm wavelength is 2.56, indicating that the prepared manganese selenide is The ma...

Embodiment 2-8

[0021] making process:

[0022] Mn 2+ The soluble salt is dissolved in deionized water to prepare a solution with a concentration of 0.05-0.08mol / L, then the aqueous solution and glycerol are mixed in a ratio of 6:1 by volume, and a certain amount of sodium lauryl sulfate is added after mixing to make The concentration of sodium dodecyl sulfate is 20~40g / L, and then a certain amount of SeO is added. 2 , making SeO 2 The concentration is 0.02~0.04mol / L, mixed with SeO 2 The aqueous solution of glycerol and hydrazine hydrate is then configured into a mixed solution in a ratio of 7:1 by volume. The mixed solution is crystallized at 160-200°C for 24-48 hours, washed with deionized water and ethanol, filtered, and the filter cake is dried at 40-60°C for 24 hours to obtain a manganese selenide mimetic enzyme whose chemical composition is MnSe Materials (see Table 1).

[0023] Accurately weigh 0.01g of manganese selenide, add 1mL of ultrapure water, shake evenly, take 200μL and ...

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Abstract

The invention relates to a mimetic enzyme technology, in particular to a manganese selenide mimetic enzyme material and the preparation and application thereof. The manganese selenide mimetic enzyme material is prepared by hydrothermal synthesis. The prepared manganese selenide mimetic enzyme material has the catalytic activity of peroxidase.

Description

technical field [0001] The present invention relates to the technology of imitating enzyme, in particular to an imitating enzyme material and its preparation and application. Background technique [0002] Peroxidase (Horseradish peroxidase, HRP for short) is a type of oxidoreductase produced by microorganisms or plants, and it uses hydrogen peroxide as an electron acceptor to catalyze the oxidation of substrates. HRP is one of the most commonly used tool enzymes in immunoenzyme labeling technology, and is often used to label antibodies. [0003] The literature (Nature Nanotechnology, 2007, 2, 577-583) first reported Fe 3 O 4 Magnetic nanoparticles have HRP catalytic activity, and their catalytic efficiency, mechanism and substrate specificity are all the same as HRP. And compared with HRP, it also has the advantages of high stability, simple preparation process and recyclability. in Fe 3 O 4 After that, it was successively reported that graphene oxide, carbon nanotubes...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01B19/04B01J23/34
CPCB01J27/0573C01B19/007C01P2002/72
Inventor 王毅张盾相振波
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI