Method for accelerating formation of biofilm in waste water under low temperature condition

A low-temperature condition and biofilm technology, which is applied in sustainable biological treatment, chemical instruments and methods, biological water/sewage treatment, etc., can solve the problem of long period of biofilm formation in wastewater, accelerated biofilm formation in wastewater, and poor firmness. High-level problems, to achieve the effect of shortening the film-hanging time, strong impact load resistance, and short film-hanging time

Active Publication Date: 2017-11-24
NANJING UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] Aiming at the problems of long cycle and low firmness of wastewater biofilm under low temperature conditions,

Method used

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  • Method for accelerating formation of biofilm in waste water under low temperature condition
  • Method for accelerating formation of biofilm in waste water under low temperature condition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A method for accelerating wastewater biofilm formation under low temperature conditions, comprising the steps of:

[0041] Vacuum freeze-dried Sphingomonas rubrum recovery culture: Use 75% alcohol absorbent cotton to sterilize the outer surface of the ampoule containing the freeze-dried bacteria and heat it with a flame, then knock off the top of the ampoule with a file or tweezers; then aseptic Take 0.5mL of liquid culture medium with a straw, drop it into the ampoule bottle whose top has been broken and shake it gently, so that the freeze-dried bacteria dissolve and form a suspension, then absorb all the bacterial suspension, and transplant it into a test tube containing 5mL of medium to keep the temperature For cultivation, the conditions for constant temperature cultivation are: tryptone soybean broth medium with a pH of 7.4, cultured on a shaker at a rotation speed of 120 r / min and at 30° C. for 36 hours.

[0042] (1) Sphingomonas rubrum is transferred to an Erlenm...

Embodiment 2

[0049] A method for accelerating wastewater biofilm formation under low temperature conditions, comprising the steps of:

[0050] Vacuum freeze-dried Sphingomonas rubrum recovery culture: Use 75% alcohol absorbent cotton to sterilize the outer surface of the ampoule containing the freeze-dried bacteria and heat it with a flame, then knock off the top of the ampoule with a file or tweezers; then aseptic Pick up 0.3mL of liquid culture medium with a straw, drop it into the ampoule bottle whose top has been broken and shake it gently, so that the freeze-dried bacteria dissolve and form a suspension. For cultivation, the conditions for constant temperature cultivation are: tryptone soybean broth medium with a pH of 7.4, cultured on a shaker at 150 r / min and 28° C. for 30 h.

[0051] (1) Sphingomonas rubrum is transferred to an Erlenmeyer flask containing sterilized liquid medium for expanded culture. The conditions for expanded culture are: tryptone soybean broth medium with a pH ...

Embodiment 3

[0059] In this case, by adding low-temperature-resistant immobilized Sphingomonas rubrum pellets to the MBBR reactor at a low temperature of 5°C, we compared the addition and non-dosing of low-temperature-resistant immobilized Sphingomonas rubrum Differences in MBBR reactor film hanging time, COD, ammonia nitrogen removal rate, and biofilm extracellular polymeric material (EPS) of pellets. Wherein the recovery culture of Sphingomonas rubrum, the expanded culture and the preparation of low temperature-resistant immobilized Sphingomonas rubrum pellets are basically the same as those in Example 2.

[0060] Table 1 shows the comparison of the film formation time, COD, ammonia nitrogen removal rate, carrier biomass, and biofilm dehydrogenase in the two sets of devices. The two sets of devices were co-cultured for 35 days from the addition of the suspension filler. Among them, device A did not add low-temperature-resistant immobilized Sphingomonas rubrum pellets at the initial stag...

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Abstract

The invention discloses a method for accelerating formation of a biofilm in waste water under a low temperature condition, and belongs to the technical field of waste water treatment. The method comprises the following steps: 1, amplification culture and low-temperature adaptation culture of a red sphingosine pure monad; 2, preparation of a low-temperature resistance immobilized red sphingosine monad pellet; 3, adding the prepared low-temperature resistance immobilized red sphingosine monad pellet, paddings, aerobic activated sludge and low-temperature waste water into an MBBR (moving bed biofilm reactor) and then performing post-aeration treatment; 4, keeping a corresponding aeration quantity and a corresponding pH value in the MBBR until the formation of the biofilm is completed. According to the method, low-temperature adaptation culture is performed on the red sphingosine monad, and the red sphingosine pure monad is added into the reactor for increasing bacterial extracellular polymers, so that microorganisms can grow on the paddings faster, and the microbial activity is improved. Therefore, the formation of the biofilm in the waste water under the low temperature condition is accelerated, and the formed biofilm is firm, strong in shock load resistance, and good in waste water treatment effect.

Description

technical field [0001] The invention belongs to the technical field of wastewater treatment, in particular to a method for accelerating biofilm formation in wastewater under low temperature conditions. Background technique [0002] With the continuous development of sewage biological treatment technology, biofilm method has been widely used, among which "moving bed biofilm reactor" (referred to as MBBR reactor) is the most widely used and most efficient treatment technology in biofilm technology. Domestic sewage, food wastewater, papermaking wastewater, petroleum wastewater, pharmaceutical wastewater and other industrial wastewater have been widely used. [0003] The MBBR reactor is a process that combines the activated sludge method of suspended growth and the biofilm method of attached growth. The reactor uses light and fine suspended fillers to provide carriers for the growth of microorganisms to increase the biomass in the reactor. Therefore, the treatment efficiency of...

Claims

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Application Information

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IPC IPC(8): C02F3/12C02F3/34C12N11/04C12N11/10
CPCC02F3/1268C02F3/34C02F2305/06C12N11/04C12N11/10Y02W10/10
Inventor 任洪强傅慧敏丁丽丽王瑾丰
Owner NANJING UNIV
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