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Method for enhancing escherichia coli resisting performance of polyethyleneimine

A polyethyleneimine, Escherichia coli technology, applied in the field of antibacterial, can solve the problems of increased cytotoxicity, limitation, unsatisfactory antibacterial effect of Escherichia coli, etc., and achieves the effects of increasing the bonding opportunity, short reaction time, and excellent antibacterial performance.

Active Publication Date: 2017-11-28
西安唐隆国际酒店有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that the antibacterial effect of B-PEI on Staphylococcus aureus is much better than that on Escherichia coli, but the unsatisfactory antibacterial effect of B-PEI on Escherichia coli limits its application; As the molecular weight of B-PEI increases, the cytotoxicity also increases

Method used

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  • Method for enhancing escherichia coli resisting performance of polyethyleneimine
  • Method for enhancing escherichia coli resisting performance of polyethyleneimine
  • Method for enhancing escherichia coli resisting performance of polyethyleneimine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Add 1g of B-PEI with a weight-average molecular weight of 600 to 7mL of 10mM pH=7.4 PBS buffer, stir well, then add 0.09g of mannose, stir at 90°C for 40min, and place it in a dialysis bag for 24h after cooling. Man-PEICPs were obtained and stored in the refrigerator at 4°C.

Embodiment 2

[0029] Add 1g of B-PEI with a weight average molecular weight of 600 to 7mL of 10mM pH=7.4 PBS buffer, stir well, then add 0.18g of mannose, stir for 40min at 90℃, and place it in a dialysis bag for 24h after cooling. Man-PEICPs were obtained and stored in the refrigerator at 4°C.

Embodiment 3

[0031] Add 1g of B-PEI with a weight average molecular weight of 600 to 7mL of 10mM pH=7.4 PBS buffer, stir well, then add 0.36g of mannose, stir for 40min at 90℃, and place it in a dialysis bag for 24h after cooling. Man-PEICPs were obtained and stored in the refrigerator at 4°C.

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Abstract

The invention discloses a method for enhancing the escherichia coli resisting performance of polyethyleneimine. According to the method, mannose is decorated on the branching type polyethyleneimine, the decoration method is simple, the reaction time is short, the cost is low, and the minimum bacteriocidal concentration of polyethyleneimine decorated with the mannose to escherichia coli is 10 [mu]g / mL, and is reduced by 22 times compared with the minimum bacteriocidal concentration (220 [mu]g / mL) of the polyethyleneimine to the escherichia coli. And meanwhile, after HeLa cells added with 500 [mu]g / mL of antibacterial agents are cultured for 24 h, the activity of the cells added with the polyethyleneimine decorated with the mannose is maintained at 75%, and the activity of the cells added with the polyethyleneimine is decreased to 56%. The method for enhancing the escherichia coli resisting performance of the polyethyleneimine obviously enhances the sterilizing rate of the polyethyleneimine to the escherichia coli and reduces the cytotoxicity.

Description

Technical field [0001] The invention belongs to the field of antibacterial technology, and specifically relates to a method for improving the anti-E. coli performance of polyethyleneimine. Background technique [0002] Escherichia coli is a common pathogenic bacterium in life that often causes diarrhea and sepsis. It is a kind of Gram-negative Brevibacterium with blunt round ends, peripheral flagella, can move, and has no spores. Distributed in nature, food is highly likely to be contaminated by it, and it is easy to cause food poisoning. For example, spinach in the United States was contaminated by E. coli in 2006, and the disease spread to half of the United States; the "poisonous cucumber" incident in Europe in 2011 was also caused by infections caused by E. coli contaminated vegetables. E. coli has caused many large-scale infections around the world, causing many deaths. In order to ensure food safety, consumers can buy green and healthy food with peace of mind. It is of g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/54A61K31/785A61P31/04
CPCA61K31/785Y02A50/30
Inventor 刘梅李娇李宣仪
Owner 西安唐隆国际酒店有限公司
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