A kind of patoside e and its preparation method and application

Abstract

The invention relates to semen lepidii cardiac glycoside E as well as a preparation method and application thereof, which are used for effectively solving the problem of extracting semen lepidii cardiac glycoside E from semen lepidii and applying to prepare a medicine for treating injury of myocardial cells H9c2. The preparation method comprises the following steps of plainly frying semen lepidii, adding water to decoct and boil and extract, concentrating an extracting solution, setting by alcohol, concentrating a supernatant until the flavor of alcohol does not exist, applying a Dianion HP-20 column, eluting by water and ethyl alcohol in a gradient way, collecting 60% ethyl alcohol eluting liquid, dissolving by water, centrifuging, dissolving precipitate by methyl alcohol, stirring a sample by silicon gel, eluting by dichloromethane and methyl alcohol as flowing phases in the gradient way, collecting the 5:1 flowing part of dichloromethane and methyl alcohol, dissolving by water, applying an ODS (octadecylsilyl) column chromatography, eluting by water, separating the eluting liquid by semi-prepared HPLC (high performance liquid chromatography), and applying a YMC-Pack ODS-AQ chromatographic column, wherein the flowing phases are acetonitrile and water with ratio of 10 to 90; collecting the flowing part with remaining time tR of 20.7 to 30.0min, concentrating, and drying, so as to obtain the semen lepidii cardiac glycoside E. The semen lepidii cardiac glycoside E has the advantages that the rich raw materials are contained, and the preparation method is scientific and reasonable; the compound has protection function on the myocardial cells, and can be used for effectively preparing the medicine for treating the injury of the myocardial cells H9c2.

Description

technical field [0001] The present invention relates to medicine, in particular to a kind of buraxin E and its preparation method and application. Background technique [0002] Tinglizi is a commonly used Chinese herbal medicine. It was first recorded in "Shen Nong's Materia Medica", and it was listed as a herbal inferior product. The medicine tastes pungent and bitter, and is very cold in nature. The "Tinglizi" included in the 2015 edition of "Chinese Pharmacopoeia" has a north-south distinction. Among them, the dry mature seeds of the cruciferous plant Lepidium apetalum Willd. .) The dry mature seeds of Webbex Prantl. are commonly called "Nan Ting Li Zi". [0003] From the research experiment, it is found that Tinglizi contains a kind of rhamnoside compound, that is, Bastariside E, but how to extract this kind of compound from Tinglizi and effectively use it in the preparation of therapeutic Drugs for cardiomyocyte H9c2 injury have not been publicly reported so far. Co...

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Problems solved by technology

[0004] In view of the above situation, in order to overcome the defects of the prior art, the purpose of the present invention is to provide a kind of burdockside E and its preparation method and application, which can effectively solve the problem of extracting burdock glycoside E from the fruit of Tinglizi and its use in Problems in preparing drugs for treating cardiomyocyte H9c2 injury

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