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Polypeptide PIP-14 for antagonizing ribonucleic acid (RNA) binding activity of protein PARP1, and application of polypeptide HIP-13

A technology that combines activity and protein, applied in the direction of peptides, hybrid peptides, peptide/protein components containing positioning/targeting motifs, etc., to achieve the effect of inhibiting tumor activity

Active Publication Date: 2017-12-08
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Peptides targeting the WGR domain of PARP1 protein have not been reported yet

Method used

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  • Polypeptide PIP-14 for antagonizing ribonucleic acid (RNA) binding activity of protein PARP1, and application of polypeptide HIP-13
  • Polypeptide PIP-14 for antagonizing ribonucleic acid (RNA) binding activity of protein PARP1, and application of polypeptide HIP-13
  • Polypeptide PIP-14 for antagonizing ribonucleic acid (RNA) binding activity of protein PARP1, and application of polypeptide HIP-13

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Embodiment Construction

[0029] The principles and features of the present invention are described below in conjunction with examples, which are only used to explain the present invention and are not intended to limit the scope of the present invention.

[0030] 1. Synthesis of Antitumor Peptides

[0031] Synthesize an anti-tumor polypeptide by solid-phase synthesis, which includes a tumor cell killing domain and a membrane-penetrating domain, wherein the tumor cell killing domain sequence is shown in SEQ ID NO: 1, and the membrane-penetrating domain sequence is as shown in SEQ ID NO: 1 As shown in ID NO: 2, it is connected to the N-terminal of the tumor cell killing domain, and the obtained sequence is: the amino acid sequence is YGRKKRRQRRR-DIVKGTNSYYKLQK (SEQ ID NO: 3), and it is named PIP-14. For the convenience of research, we linked FITC labeled with fluorescein isothiocyanate to the C-terminus of the anti-tumor polypeptide, and linked biotin-labeled Biotin to the N-terminus.

[0032] 2. Detect...

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Abstract

The invention provides a polypeptide for antagonizing the ribonucleic acid (RNA) binding activity of a protein PARP1, and application of the polypeptide. The amino acid sequence of the polypeptide is as shown in SEQ ID No.1; the invention also relates to an anti-tumor polypeptide and application thereof; the anti-tumor polypeptide comprises a tumor cell kill structure domain and a transmembrane structure domain, and the amino acid sequence of the tumor cell kill structure domain is as shown in SEQ ID No.1. The transmembrane structure domain of the anti-tumor polypeptide per se does not have cytotoxicity, but has obvious effects of inhibiting tumor proliferation and migration attack after being connected with the tumor cell kill structure domain. The anti-tumor polypeptide provided by the invention not only can be used as an anti-tumor biological treatment drug alone, but also is expected to be combined with other treatment ways for inhibiting tumors.

Description

technical field [0001] The present invention relates to the field of tumor targeting therapy, and more particularly relates to a polypeptide capable of antagonizing the RNA binding activity of PARP1 protein and its application. Background technique [0002] Tumor seriously affects human health and is a major cause of death worldwide. Although the existing anti-tumor methods have a certain effect on most tumors, they still have problems such as low curative effect, poor selectivity, high toxicity and side effects, and easy drug resistance. Therefore, searching for new anti-tumor drugs with high efficiency and low toxicity has always been a hot spot in the research and development of medicines at home and abroad. [0003] Targeted tumor therapy refers to targeting tumor cells with drugs at the molecular level to make them die specifically without affecting normal tissue cells. Unlike traditional cytotoxic chemotherapy, tumor molecular targeted therapy has a specific anti-tum...

Claims

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Application Information

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IPC IPC(8): C07K7/08C07K19/00A61K38/10A61K47/42A61P35/00
CPCA61K38/00C07K7/08C07K2319/10
Inventor 童强松郑丽端方二虎王晓静宋华杰杨枫叶霖李聃
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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