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Lycopene fermentation production method through microcirculation spore production mechanism

A microcirculation spore production and lycopene technology, applied in the biological field, can solve problems such as accelerating the development of inoculation points, and achieve the effects of low cost, accelerated development and simple operation

Inactive Publication Date: 2017-12-15
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Microcirculatory sporulation leads to a high number of conidia formed through dentition, accelerating the development of the inoculation site

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Step 1: Activation of strains.

[0020] Prepare PDA medium, take 200g of peeled potatoes, cut into small pieces, add 1000ml of water to boil for 20-30 minutes, filter with eight layers of gauze to obtain the filtrate. Add 20 g of glucose and 16 g of agar to the filtrate, heat and dissolve and then set the volume to 1000 milliliters, dispense them into test tubes, and tilt them while they are hot to obtain PDA medium. Streaks of N. crassa were taken and cultured under light at 30°C for 48 hours.

[0021] Step 2: Fermentation culture.

[0022] Fermentation medium: glucose 30g / L; peptone 20g / L; NaNO 3 3.0g / L; MgSO 4 0.5g / L; KCl0.5g / L; FeSO 4 0.01g / L; K 2 HPO 4 1.0g / L; agar powder 16g / L; after heating and dissolving, dilute to 1L with distilled water, natural pH. Use an inoculating loop to pick two rings of Neurobacillus spores and inoculate them into an Erlenmeyer flask filled with 30 mL of sterile water, and shake the flask at 30°C and 120 r / min for 1 hour to dispe...

Embodiment 2

[0028] Step 1: Activation of strains (same as Example 1).

[0029] Step 2: Fermentation culture.

[0030] Fermentation medium: glucose 30g / L; peptone 20g / L; NaNO 3 3.0g / L; MgSO 4 0.5g / L; KCl0.5g / L; FeSO 4 0.01g / L; K 2 HPO 4 1.0g / L; agar powder 16g / L; after heating and dissolving, dilute to 1L with distilled water, natural pH. Use an inoculating loop to pick two rings of Neurobacillus spores and inoculate them into an Erlenmeyer flask filled with 30 mL of sterile water, and shake the flask at 30°C and 120 r / min for 1 hour to disperse the spores in the liquid evenly. Then inoculate 1% of the inoculum into plates containing 6 15mL solid fermentation medium. Three of the culture media were cultured under light at 43°C for 17 hours, and then transferred to 25°C for 36 hours under light. The other three mediums were cultured under light at 30°C for 53 hours.

[0031] Assay.

[0032] Assay method is the same as in Example 1. Results: The average content of the experimental ...

Embodiment 3

[0034] Step 1: Activation of strains (same as Example 1).

[0035]Step 2: Fermentation culture.

[0036] Fermentation medium: glucose 30g / L; peptone 20g / L; NaNO 3 3.0g / L; MgSO 4 0.5g / L; KCl0.5g / L; FeSO 4 0.01g / L; K 2 HPO 4 1.0g / L; agar powder 16g / L; after heating and dissolving, dilute to 1L with distilled water, natural pH. Use an inoculation loop to pick two rings of Neurobacillus crassa spores and inoculate them into an Erlenmeyer flask filled with 30 mL of sterile water, shake the flask at 30°C and 120 r / min for 1 hour, so that the spores in the liquid are evenly dispersed. Then inoculate 1% of the inoculum into plates containing 6 15mL solid fermentation medium. Three of the culture media were cultured under light at 43°C for 20 hours, and then transferred to 25°C for light culture for 48 hours. The other three mediums were cultured under light at 30°C for 68 hours.

[0037] Step 3 content determination.

[0038] After the fermentation is over, collect the spores...

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PUM

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Abstract

The invention discloses a lycopene fermentation production method through a microcirculation spore production mechanism. The method includes the steps that neurospora crassa is activated and cultivated for 48 hours in a solid PDA cultivation medium at the temperature of 30 DEG C to be subjected to fermentation cultivation; the product is subjected to illumination cultivation for 10 hours to 24 hours at the temperature of 40 DEG C to 46 DEG C, and then is transferred at the temperature of 20 DEG C to 30 DEG C and continues to be subjected to illumination cultivation for 24 hours to 48 hours; after fermentation is completed, spores are collected and dried, and then dry thallus rich in lycopene is obtained. In the cultivation condition, the neurospora crassa generates conidia rich in lycopene through the microcirculation spore production mechanism. According to the lycopene fermentation production method, the lycopene fermentation production period of the neurospora crassa is effectively shortened through the microcirculation spore production mechanism of the neurospora crassa, and the yield of the lycopene is increased. The method is easy and convenient to operate, the condition is stable, control is easy, and the cost is low.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to fermentation engineering, in particular to a method for producing lycopene by fermentation using a microcirculation sporulation mechanism. Background technique [0002] Lycopene is an important carotenoid, which has physiological functions such as anti-oxidation, anti-aging, improving immunity, preventing heart disease, slowing down atherosclerosis, preventing various cancers, protecting cardiovascular and cerebrovascular, etc. , has broad application prospects and high commercial value. Lycopene cannot be synthesized by the human body and must be obtained through diet. With the continuous improvement of people's living standards and health awareness, lycopene products are gradually being accepted by the market and consumers. According to the forecast of Customer Management Relations International Company (CMR) in the United States, the sales of lycopene products will increase ever...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P5/02C12N3/00C12R1/645
CPCC12N3/00C12P5/007
Inventor 陈钢阙发秀
Owner NANCHANG UNIV