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Umbilical cord mesenchymal stem cells extract product and application of umbilical cord mesenchymal stem cells extract product on beauty treatment

A technology of stem cells and extracts, applied in the field of anti-aging facial fillers for beauty, can solve the problems of skin anti-aging repair beauty products, damage to stem cell characteristics, wrong signals, etc., to promote hematopoietic recovery function and maintain the effect Long-lasting, excellent results

Inactive Publication Date: 2017-12-19
HENAN YINFENG BIOENG CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Increased survival time and number of replications lead to accumulation of faulty signals at the genetic level within stem cells, meaning that even when these cells can be obtained and expanded, their stem cell properties are compromised
[0005] At present, there is no beauty product for skin anti-aging repair using umbilical cord mesenchymal stem cells

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A kind of umbilical cord mesenchymal stem cell extract, it is extracted by following steps:

[0023] (1) Umbilical cord mesenchymal stem cells are isolated from umbilical cord blood;

[0024] (2) Obtaining umbilical cord mesenchymal stem cells in step (1) using serum-free culture medium, and isolating umbilical cord mesenchymal stem cells and culture medium;

[0025] (3) lysing the umbilical cord mesenchymal stem cells, and adding culture medium to obtain protein suspension;

[0026] (4) Concentrating the protein suspension obtained in step (3) to obtain a concentrate, which is the umbilical cord mesenchymal stem cell extract.

[0027] In step (1), the preparation method of umbilical cord mesenchymal stem cells is:

[0028] Take the umbilical cord blood, mix it with the same amount of PBS buffer, slowly drop into the same amount of lymphocyte separation medium, and centrifuge at 650g for 15 minutes; extract the buffy coat cells, wash and centrifuge with the same amoun...

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PUM

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Abstract

The invention discloses an umbilical cord mesenchymal stem cells extract product and an application of the umbilical cord mesenchymal stem cells extract product on beauty treatment. An extraction method of the umbilical cord mesenchymal stem cells extract product comprises the following steps: 1) separating the umbilical cord mesenchymal stem cells from umbilical cord blood to obtain the umbilical cord mesenchymal stem cells; 2) using a serum-free medium for culturing the umbilical cord mesenchymal stem cells obtained in the step 1), and separating the umbilical cord mesenchymal stem cells and a nutrient solution; 3) cracking the umbilical cord mesenchymal stem cells, and adding the nutrient solution to obtain a protein suspension; and 4) concentrating the protein suspension obtained in the step 3) to obtain a concentrate which is the umbilical cord mesenchymal stem cells extract product. The umbilical cord mesenchymal stem cells have strong immunoloregulation effect, promote hematopoiesis recovery function, and recover the damaged or pathological tissue organs; the umbilical cord mesenchymal stem cells extract product is used for a beauty treatment product, and has the advantages of excellent effect after usage and long effect maintenance time.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an anti-aging facial filler for cosmetic use using umbilical cord mesenchymal stem cells. Background technique [0002] Umbilical cord mesenchymal stem cells (Mesenchymal Stem Cells, MSCs) refer to a kind of multifunctional stem cells existing in the umbilical cord tissue of newborns, which can differentiate into many kinds of tissue cells and have broad clinical application prospects. [0003] Umbilical cord mesenchymal stem cells have high differentiation potential and can differentiate in multiple directions. It has broad clinical application prospects in tissue engineering of bone, cartilage, muscle, tendon, ligament, nerve, liver, endothelium and cardiac muscle. It has been reported that mesenchymal stem cells were isolated from human umbilical cord, and the cell content and proliferation ability were superior to those of bone marrow mesenchymal stem cells, the immu...

Claims

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Application Information

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IPC IPC(8): C12N5/0775C12N1/06A61K8/99A61Q19/08
CPCA61K8/99A61K2800/10A61Q19/08C12N1/06C12N5/0665
Inventor 朱学义徐峰波李雯雯王秋文程志远王彦
Owner HENAN YINFENG BIOENG CO LTD