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Riemerella anatipestifer infection attenuated live virus vaccine and preparation method thereof

A technique for Riemerella anatipestifer and Mergerella anatipestifer is applied in the field of Riemerella anatipestifer live attenuated vaccine and preparation thereof, and achieves the effects of good immunity, improved resistance and good safety.

Inactive Publication Date: 2017-12-26
INST OF ANIMAL HEALTH GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no domestic report on the research on the attenuated live vaccine of Riemerella anatipestifer

Method used

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  • Riemerella anatipestifer infection attenuated live virus vaccine and preparation method thereof
  • Riemerella anatipestifer infection attenuated live virus vaccine and preparation method thereof
  • Riemerella anatipestifer infection attenuated live virus vaccine and preparation method thereof

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Embodiment 1

[0029] Embodiment 1 Riemerella anatipestifer GD strain

[0030] 1. The source and preservation of the strain

[0031] The strain used for the production of the vaccine is Flavobacteriaceae, a new genus of Riemerella, and Riemerella anatipestifer GD strain, which was isolated by the inventor from the heart of ducklings in a duck farm in Guangdong Province in 2012, and was obtained in 2016. On April 1, it was sent to the China Center for Type Culture Collection for preservation, with the preservation number: CCTCC NO: M 2016161. It is identified by Gram staining, PCR technology, animal regression test, biochemical test and other methods.

[0032] 2. Strain characteristics

[0033] (1) Morphology of Riemerella anatipestifer GD strain

[0034] The heart of the duck was taken aseptically, streaked and inoculated on TSA plate and MacConkey agar plate, cultured in a candle jar at 37°C for 18-24 hours, and a single suspicious colony was picked for pure culture. The bacterium grew ...

Embodiment 2

[0057] Embodiment 2 Riemerella anatipestifer attenuated live vaccine and preparation method thereof

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Abstract

The invention discloses a riemerella anatipestifer infection attenuated live virus vaccine and a preparation method thereof. The riemerella anatipestifer infection attenuated live virus vaccine disclosed by the invention contains a riemerella anatipestifer GD strain freeze-drying protection agent and a riemerella anatipestifer GD strain is preserved in China Center for Type Culture Collection (CCTCC) on April 1, 2016, with the preservation number of CCTCC NO: M2016161. The riemerella anatipestifer infection attenuated live virus vaccine disclosed by the invention has good safety and does not have any adverse effect on ducks; the riemerella anatipestifer infection attenuated live virus vaccine has a good immune effect, can be used for remarkably improving the resistance of young ducks on riemerella anatipestifer, can be used for clinically preventing riemerella anatipestifer infection and has important economic and social benefits.

Description

technical field [0001] The invention belongs to the field of animal vaccines, and relates to an attenuated live vaccine of Riemerella anatipestifer and a preparation method thereof. Background technique [0002] Riemerella anatipestifer, also known as duck infectious serositis, new duck disease, and duck plague syndrome, is a contact acute or chronic septicemia caused by Riemerella anatipestifer (RA). Infectious disease, mainly affecting ducklings aged 2-7 weeks, clinically manifested as increased eye and nasal secretions, panting, coughing, diarrhea, ataxia, and head and neck tremors, and chronic diseased ducks mainly manifested as meningitis Symptoms, neck tilt, slow growth or stiff ducks, lesions are characterized by fibrinous pericarditis, perihepatitis and air sacs, and the mortality rate is as high as 90%, causing huge economic losses to the duck industry. The survey shows that Riemerella anatipestifer has occurred in all parts of our country. Due to the diversity an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K39/02A61P31/04C12R1/01
CPCA61K39/0208A61K2039/522A61K2039/552A61P31/04C12N1/20C12N1/205C12R2001/01
Inventor 吴彩艳董嘉文吕敏娜廖申权李林林孙敏华张建峰孙铭飞徐志宏
Owner INST OF ANIMAL HEALTH GUANGDONG ACADEMY OF AGRI SCI
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