Test strip for detecting HIV (Human Immunodeficiency Virus) antibody in urea, detection cup and preparation method of test strip

Abstract

The invention discloses a test paper strip for detecting HIV antibody in urine, a detection cup and a preparation method thereof. The test paper strip includes a bottom plate, a sample pad, a nitrocellulose membrane and water-absorbing paper arranged on the bottom plate. The two ends of the nitrocellulose membrane are overlapped with the sample pad and absorbent paper respectively, and the mouse anti-human IgG antibody labeled with colloidal gold and the gp160 antigen labeled with colloidal gold are sprayed on the sample pad near the end of the nitrocellulose membrane. , the nitrocellulose membrane is provided with the first detection line coated with HIV recombinant antigen gp41 and HIV recombinant antigen gp160, and the control line coated with goat anti-mouse antibody; the test strip of the present invention is added with new antigen gp160, Compared with the current anti-gp41 detection method, the occurrence of missed detection can be reduced; the high-sensitivity urine test of the test strip for detecting HIV antibody in urine of the present invention ensures the authenticity and reliability of the data, and can be used for large-scale primary screening of AIDS.

Description

technical field [0001] The invention belongs to the technical field of medicine, and more specifically, the invention relates to a test strip for detecting HIV antibodies in urine, a detection cup and a preparation method thereof. Background technique [0002] Human immunodeficiency virus (Human Immunodeficiency Virus, HIV) is a virus that infects the cells of the human immune system. It destroys the immune cells of the human body and causes the immune system to lose its resistance, resulting in various diseases. According to statistics from the National Center for Disease Control and Prevention, since 2007, AIDS has become the infectious disease that has caused the highest death toll in China and continues to this day. It is worth noting that 32.1% of the infected people in China are still undetected. The only way to determine whether a person has HIV is to get an HIV test. Data show that the number of HIV tests in China increased rapidly between 2008 and 2015. According...

AI Technical Summary

Problems solved by technology

The only non-infectious / very infectious urine testing method approved by CFDA is ELISA, which takes a long time (3 hours) and can only be used for qualitative testing

The colloidal gold method, which can also be used for qualitative detection, is favored and considered as the first choice because of its simple operation and short time-consuming. However, due to the low urine antibody content and sensitivity problems, no similar products have been found in the existing market. In addition, , it has been reported that compared with blood, urine testing has a higher false positive problem in saliva

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