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Compound nano preparation for treating T cell acute lymphoblastic leukemia, preparation method and application

An acute lymphocyte and nano preparation technology is applied in the preparation of compound nano preparations. In the field of preparation of drugs for the treatment or prevention of T-cell acute lymphoblastic leukemia, compound nano preparations can solve the problems of increased toxic and side effects of healthy cells and prolong survival rate. , Easy to use, reasonable formula effect

Active Publication Date: 2018-01-09
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the cytotoxic effect on malignant T-ALL cells was improved by the combination of IRAK1 / 4 inhibitor and ABT-737, the corresponding toxic side effect on healthy cells was also increased at the same time
However, the systemic side effects caused by chemotherapy drugs are currently the biggest challenge and obstacle in the clinical treatment of leukemia.

Method used

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  • Compound nano preparation for treating T cell acute lymphoblastic leukemia, preparation method and application
  • Compound nano preparation for treating T cell acute lymphoblastic leukemia, preparation method and application
  • Compound nano preparation for treating T cell acute lymphoblastic leukemia, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A compound nano-preparation for the treatment of T-cell acute lymphoblastic leukemia, which is made of the following raw materials in parts by weight:

[0038]

[0039] The mixture of the IRAK1 / 4 inhibitor and ABT-737 is prepared by mixing the RAK1 / 4 inhibitor and ABT-737 at a weight ratio of 1:1.

[0040] The mass volume concentration of the polylactic acid-polyglycolic acid is 2.98%.

[0041] The mass volume concentration of the polyvinyl alcohol solution is 2%.

[0042] A method for preparing a compound nano-preparation for the treatment of T-cell acute lymphoblastic leukemia, the steps of which are:

[0043] (1) Dissolve a certain amount of polylactic acid-polyglycolic acid (PLGA) polymer (Evonik Industries, Ltd.), IRAK1 / 4 inhibitor and ABT-737 in 3 mL of acetonitrile solution to obtain a mixed solution;

[0044] (2) The mixed solution that step (1) obtains is emulsified in the polyvinyl alcohol solution of 12mL2% (w / v) to form emulsion, uses the microprobe sonic...

Embodiment 2-6

[0050] A compound nano-preparation for the treatment of T-cell acute lymphoblastic leukemia, which is made of the following raw materials in parts by weight:

[0051]

Example 2

Example 3

Example 4

Example 5

Example 6

Mixture of RAK1 / 4 inhibitors and ABT-737

1.5

2.5

3.5

4.5

5.5

polylactic acid-polyglycolic acid

52

70

80

100

40

Acetonitrile solution

1

2

3

4

5

polyvinyl alcohol solution

12

24

24

45

45

[0052] Its preparation steps are identical with embodiment 1.

[0053] Characterization analysis:

[0054] Dynamic light scattering (DLS) analyzes the IRAK / ABT-NP that embodiment 1-6 step (4) obtains, and the result shows, the average diameter of IRAK / ABT-NP is 100nm, monodisperse size distribution; By SEM and TEM studied surface morphology, indicating that the particles are spherical (see figure 1 ).

[0055] The IRAK / ABT-NP in vitro release test that embod...

Embodiment 7

[0067] An application of a compound nano-preparation in the preparation of a medicine for treating or preventing T-cell acute lymphoblastic leukemia, the steps of which are:

[0068] A, the dry compound nano powder preparation is dispersed in saline for injection, the concentration is 2mg / mL;

[0069] B. Vibrate on a vortex to make the nanosuspension fully mixed, and then intravenously inject or drip.

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Abstract

The invention discloses a compound nano preparation for treating T cell acute lymphoblastic leukemia, a preparation method and application. The nano preparation is prepared from a mixture of an inhibitor and ABT-737 in a certain proportion, polylactic acid-polyglycolic acid, acetonitrile and polyvinyl alcohol, the preparation method comprises the steps: (1) dissolving the polylactic acid-polyglycolic acid polymer, the IRAK1 / 4 inhibitor and the ABT-737 in an acetonitrile solution to obtain a mixed solution; (2) emulsifying the mixed solution in a polyvinyl alcohol solution to form an emulsion and utilizing a micro probe ultrasonoscope for emulsifying on ice bath to obtain an emulsion; (3) stirring the emulsion under room temperature to obtain the inhibitor and compound nano particles; (4) centrifuging the compound nano particles in overspeed in an ultra centrifuge, washing by distilled water and freeze drying and storing to obtain the inhibitor and the nano particles. The invention further relates to the application of the compound nano preparation. The compound nano preparation has a reasonable formula and convenience in use, prolongs medicine metabolism time, increases medicine absorption in tumor cells and further enhances a synergistic effect of medicine.

Description

technical field [0001] The invention belongs to the technical field of compound nano preparations, in particular to a compound nano preparation drug for treating T-cell acute lymphoblastic leukemia, and also relates to a preparation method of a compound nano preparation for treating T-cell acute lymphoblastic leukemia, and also relates to The invention relates to the use of a compound nano-preparation in the preparation of medicines for treating or preventing T-cell acute lymphoblastic leukemia. Background technique [0002] T-cell acute lymphoblastic leukemia (T-ALL) is caused by the clonal expansion of mutant T-cell progenitor cells and is considered a high-risk leukemia, accounting for about 15% of acute lymphoblastic leukemia in children and up to 25% in adults. With contemporary chemotherapy, complete remission rates are as high as 85% in children, but not ideal in adults. Only 15%-25% of adult T-ALL patients have a good prognosis after relapse, and the 5-year survival...

Claims

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Application Information

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IPC IPC(8): A61K9/51A61K47/34A61K47/32A61K45/06A61K31/635A61P35/02
Inventor 吴小艳
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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