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Preparation method for hybridized hydrogel material and application

A hydrogel and hybridization technology, which is applied in the field of preparation of hybrid hydrogel materials, can solve problems such as complex operating conditions, irreversibility, and undisclosed methods of constructing hybrid supramolecular hydrogel materials through electrostatic interactions

Active Publication Date: 2018-01-09
HUAIYIN TEACHERS COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the operation conditions are complex and irreversible through covalent bonding, and there is no method for constructing hybrid supramolecular hydrogel materials through electrostatic interaction.

Method used

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  • Preparation method for hybridized hydrogel material and application
  • Preparation method for hybridized hydrogel material and application
  • Preparation method for hybridized hydrogel material and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] 1. Raw materials

[0080] 1.1 Synthesis of macromolecular initiator NH 2 -Phe-PEG 227 -Phe-NH 2 raw material

[0081]

[0082] 1.2 Raw materials for the synthesis of Lys(Z)-NCA

[0083] H-Lys(Z)-OH 1g, 3.57mmol

[0084] Triphosgene 0.42g, 1.43mmol

[0085] 1.3 Raw materials for the synthesis of polylysine-polyethylene glycol-polylysine

[0086] NH 2 -Phe-PEG 227 -Phe-NH 2 1g, 0.1mmol

[0087] Lys(Z)-NCA 0.31g, 1mmol

[0088] 1.4 Raw materials for the synthesis of hybrid hydrogels

[0089] The charge concentrations of the positively charged amino groups of polylysine and the negatively charged phosphate groups in the DNA structure are respectively controlled at 20mM, and the charge molar ratio of the triblock copolymer to protist DNA is 1:16.

[0090] 2. Preparation method

[0091] 2.1 Synthesis of phenylalanine-terminated polyethylene glycol (NH 2 -Phe-PEG 227 -Phe-NH 2 )

[0092] Phenylalanine-terminated polyethylene glycol (NH 2 -Phe-PEG 227 -Ph...

Embodiment 2

[0113] 1. Raw materials

[0114] 1.1 Synthesis of macromolecular initiator NH 2 -Phe-PEG 227 -Phe-NH 2 raw material

[0115]

[0116] 1.2 Raw materials for the synthesis of Lys(Z)-NCA

[0117] H-Lys(Z)-OH 0.8g, 2.85mmol

[0118] Triphosgene 0.339g, 1.14mmol

[0119] 1.3 Raw materials for the synthesis of polylysine-polyethylene glycol-polylysine

[0120] NH 2 -Phe-PEG 227 -Phe-NH 2 0.8g, 0.08mmol

[0121] Lys(Z)-NCA 0.49g, 1.6mmol

[0122] 1.4 Raw materials for the synthesis of hybrid hydrogels

[0123] The charge concentrations of the positively charged amino groups of polylysine and the negatively charged phosphate groups in the DNA structure are respectively controlled at 20mM, and the charge molar ratio of the triblock copolymer to protist DNA is 1:10.

[0124] 2. Preparation method

[0125] 2.1 Synthesis of phenylalanine-terminated polyethylene glycol (NH 2 -Phe-PEG 227 -Phe-NH 2 ) method with embodiment 1.

[0126] 2.2 Synthesis of Lys(Z)-NCA

[012...

Embodiment 3

[0138] 1. Raw materials

[0139] 1.1 Synthesis of macromolecular initiator NH 2 -Phe-PEG 227 -Phe-NH 2 raw material

[0140]

[0141] 1.2 Raw materials for the synthesis of Lys(Z)-NCA

[0142] H-Lys(Z)-OH 1.5g, 5.35mmol

[0143] Triphosgene 0.635g, 2.14mmol

[0144] 1.3 Raw materials for the synthesis of polylysine-polyethylene glycol-polylysine

[0145] NH 2 -Phe-PEG 227 -Phe-NH 2 1.2g, 0.12mmol

[0146] Lys(Z)-NCA 0.294g, 0.96mmol

[0147] 1.4 Raw materials for the synthesis of hybrid hydrogels

[0148] The charge concentrations of the positively charged amino groups of polylysine and the negatively charged phosphate groups in the DNA structure are respectively controlled at 20mM, and the charge molar ratio of the triblock copolymer to protist DNA is 1:20.

[0149] 2. Preparation method

[0150] 2.1 Synthesis of phenylalanine-terminated polyethylene glycol (NH 2 -Phe-PEG 227 -Phe-NH 2 ) method with embodiment 1.

[0151] 2.2 Synthesis of Lys(Z)-NCA

[0...

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Abstract

The invention provides a preparation method for a hybridized hydrogel material and an application thereof at the aspects of tissue engineering, medical materials and drug load. The hybridized hydrogelmaterial is prepared by electrostatically bonding polypeptide-polyethylene glycol-polypeptide triblock copolymer with milt DNA. The method has the characteristics of low cost, mild forming conditions, responsibility, reversibility, self-healing property, and the like. The hybridized hydrogel material prepared according to the method provided by the invention has the biodegradability of the copolymer, ordered controllable structure unit of DNA and characteristics of transient network structure and is capable of solving the problem of toxicity caused by the traditionally used photo-initiator and chemical crosslinking agent.

Description

technical field [0001] The invention relates to the technical field of new materials, in particular to a preparation method and application of a hybrid hydrogel material constructed based on the electrostatic interaction between polypolypeptide-polyethylene glycol-polypeptide triblock copolymer and DNA. Background technique [0002] The hydrogel material is a gel material with water as the dispersion medium. It is a water-soluble polymer with a network cross-linked structure that introduces a part of hydrophobic groups and hydrophilic residues. The hydrophilic residues combine with water molecules to absorb water. A cross-linked polymer in which the molecules are connected within the network and the hydrophobic residues swell with water. Due to its good performance, it has wide application prospects in tissue engineering and drug loading, especially the hydrogel materials based on polypeptides. [0003] Polypeptide hydrogel materials are biodegradable and biocompatible, and...

Claims

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Application Information

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IPC IPC(8): C08J3/075C08L77/04C08G69/40C08G65/333A61L27/52A61L27/18A61L27/00A61K47/34A61K47/26
CPCA61K47/26A61K47/34A61L27/00A61L27/18A61L27/52C08G65/333C08G69/40C08J3/075C08L77/04
Inventor 陈平王翔行岳真支三军胡华友仲慧
Owner HUAIYIN TEACHERS COLLEGE
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