Preparation method and application of NHS immunomagnetic beads for capturing vibrio parahaemolyticus

Abstract

The invention relates to a preparation method of NHS immunomagnetic beads modified with an NHS group, in particular to a preparation method and an application of NHS immunomagnetic beads for capturingvibrio parahaemolyticus. According to the method, a magnetic fluid is used as a carrier and coupled with a vibrio parahaemolyticus antibody, and the vibrio parahaemolyticus immunomagnetic beads capable of enriching a target strain is prepared. With adoption of the preparation method of the NHS immunomagnetic beads, the time is shortened from 26 h in a traditional preparation process to 2-3 h, andthe preparation efficiency is improved; experimental control shows that compared with the traditionally prepared vibrio parahaemolyticus immunomagnetic beads, accuracy rate of enriched target flora is increased by 6.7 %; sensitivity of the vibrio parahaemolyticus immunomagnetic beads is improved, and by means of the characteristics that the vibrio parahaemolyticus immunomagnetic beads are quick,convenient and cheap and a large detection instrument is needless, the immunomagnetic beads can be applied to separation and enrichment of the vibrio parahaemolyticus in food, feed, medical environment and natural environment, and the vibrio parahaemolyticus can be detected efficiently and rapidly.

Description

technical field [0001] The invention relates to a preparation method of immunomagnetic beads based on NHS group modification, in particular to a preparation method and application of NHS immunomagnetic beads for capturing Vibrio parahaemolyticus, and the preparation method is used for separating pathogenic microorganisms parahaemolyticus The vibrio belongs to the technical field of pathogenic bacteria isolation. Background technique [0002] In the prior art, existing scholars have studied the preparation method of Vibrio parahaemolyticus immune magnetic beads, but there are three problems: 1. The effect of using foreign magnetic beads Dynabeads is better, but foreign magnetic beads are expensive, exceeding 10 times the price of domestic magnetic beads; 2. The type of magnetic beads is carboxyl magnetic beads or amino magnetic beads. Although these two types of magnetic beads are cheap, they need to be activated before connecting the antibody, and the magnetic beads are coup...

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Problems solved by technology

[0002] In the prior art, existing scholars have studied the preparation method of Vibrio parahaemolyticus immune magnetic beads, but there are three problems: 1. The effect of using foreign magnetic beads Dynabeads is better, but foreign magnetic beads are expensive, exceeding 10 times the price of domestic magnetic beads; 2. The type of magnetic beads is carboxyl magnetic beads or amino magnetic beads. Although these two types of magnetic beads are cheap, they need to be activated before connecting the antibody, and the magnetic beads are coupled with the antibody. It takes a long time, generally overnight, which greatly affects the efficiency of immunomagnetic beads preparation; 3. Existing research is mainly to enrich 1mL samples, which does not reflect the application advantages of immunomagnetic beads

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