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Bauhinia championii extract as well as preparation method and application thereof

A technique of extracting vines and extracts, which is applied in the field of pesticides to achieve a wide range of applications, good antibacterial activity, and good herbicidal activity

Active Publication Date: 2018-01-12
YANGTZE UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a kind of evergreen vine, Asparagus seldom suffers from diseases, and there are few weeds around it. Our analysis suggests that there may be substances resistant to diseases and weeds in Asparagus. There is no report on the inhibitory effect of the extract on the common pathogens of crops and the roots and shoots of weeds in the field

Method used

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  • Bauhinia championii extract as well as preparation method and application thereof
  • Bauhinia championii extract as well as preparation method and application thereof
  • Bauhinia championii extract as well as preparation method and application thereof

Examples

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preparation example Construction

[0016] The first aspect of the present invention provides a method for preparing an extract of Astragalus chinensis, the steps comprising:

[0017] (1) Grinding the dried leaves of Asparagus chinensis, adding methanol to extract at 15-30°C, collecting the filtrate, and concentrating to obtain the primary extract of Asparagus genus;

[0018] (2) extracting the primary extract of Asparagus chinensis with a solvent, depigmenting and desolventizing to obtain the asparagus genus extract.

[0019] Preferably, in step (1), the pulverized leaves of Asparagus japonica are sequentially extracted with anhydrous methanol for 3 to 5 times until the filtrate has no color change. The mass ratio is 3-5.

[0020] Preferably, step (3) is also included to collect the components after carrying out column chromatography separation of the Asparagus vine extract; the column chromatography adopts the eluent of two systems of sherwood oil-ethyl acetate and chloroform-methanol by The order of polarit...

Embodiment 1

[0032] This example provides an extract of Asparagus genus, the specific preparation method is as follows: at room temperature (15-30°C), cold soak and extract the leaves (300.00g) of genus asparagus dried in the shade with 1500mL of anhydrous methanol, collect and extract solution, and then continue to extract the leaves twice with 1500mL of anhydrous methanol, collect and combine the extracts, wherein, the first leaching for 5 days, the second leaching for 3 days, and the third leaching for 1 day, the extraction The solution was concentrated under reduced pressure at 60°C with a rotary evaporator to a paste, and placed in a 60°C electric constant temperature blast drying oven for further drying.

Embodiment 2

[0034] In this example, the active components of the methanol extract of Ascaria chinensis are separated and analyzed, and the methanol extract of Asparagus genus obtained in Example 1 is used for column chromatography, and the specific steps include:

[0035] (1) Mixing sample of extract

[0036] Weigh 1.5 times the sample of 200-300 mesh silica gel, dry the water in a constant temperature blower at 80°C, and place it in a desiccator to cool to room temperature. Add methanol to fully dissolve the dried sample obtained in Example 1, then concentrate under reduced pressure, pour silica gel equal in weight to the sample in a mortar, draw a drop of sample solution and lightly touch the upper surface of the dried silica gel, and observe the penetration of the solution In the case of silica gel, if it penetrates quickly, the solution needs to be further concentrated. If it cannot penetrate, it needs to be diluted with an appropriate amount of methanol until the solution can just pe...

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PUM

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Abstract

The invention provides a bauhinia championii extract as well as a preparation method and application thereof. The bauhinia championii extract is a methanol extract of bauhinia championii leaves. The bauhinia championii leaf extract has broad-spectrum bactericidal activity and weedicidal activity, and can be applied to control of diseases caused by a variety of pathogenic bacteria such as oomycetes, basidiomycetes, ascomycetes, deuteromycetes and the like on a variety of crops as well as weed damage caused by common field weeds.

Description

technical field [0001] The invention relates to the technical field of pesticides, in particular to an extract of asparagus vine and its preparation method and application. Background technique [0002] Bauhinia championii Benth, also known as Jiulongteng, Plum Blossom into the Bone Dan, Bauhinia championii Benth, Wulangteng, Guogang Yuanlong, etc., is a perennial vine of the genus Bauhinia of the family Fabaceae. It is distributed in Guangdong, Hunan, and Hubei. , Jiangxi, Fujian, Zhejiang, Guizhou, Guangxi and other places, growing in areas with an altitude of 200 meters to 1000 meters, containing flavonoids, aromatic acids, flavanols, sterols, terpenes, sugars, etc., have antibacterial , anti-inflammatory and analgesic, anti-tumor, eliminate free radicals, anti-platelet aggregation, treatment of rheumatoid arthritis, expelling wind and dampness, and treatment of epigastric pain. It is an important traditional Chinese medicine. As a kind of evergreen vine, Asparagus seldo...

Claims

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Application Information

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IPC IPC(8): C07C41/34C07C41/36C07C43/196A01N25/14A01N31/06A01P3/00A01P13/00
Inventor 吴清来姚宗理李俊凯黄冠
Owner YANGTZE UNIVERSITY
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