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Kit for aided-diagnosis and prognostic evaluation on glioblastoma based on gene CLCF1 and use method thereof

A glioblastoma, auxiliary diagnosis technology, applied in the field of prognostic evaluation kit, glioblastoma auxiliary diagnosis, can solve the problem of few clinical significance research, achieve the effect of objective evaluation and reduced survival time

Inactive Publication Date: 2018-01-12
THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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Problems solved by technology

There are few studies on the expression of CLCF1 in tumors and its clinical significance, especially in gliomas, the expression and prognostic indicators are still unknown

Method used

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  • Kit for aided-diagnosis and prognostic evaluation on glioblastoma based on gene CLCF1 and use method thereof
  • Kit for aided-diagnosis and prognostic evaluation on glioblastoma based on gene CLCF1 and use method thereof
  • Kit for aided-diagnosis and prognostic evaluation on glioblastoma based on gene CLCF1 and use method thereof

Examples

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specific Embodiment

[0034] The experimental methods in the following examples are conventional methods unless otherwise specified, and the involved experimental reagents and materials are conventional biochemical reagents and materials unless otherwise specified.

[0035] 1. TCGA database comparison of CLCF1 differential expression in LGG and GBM

[0036] The Cancer Genome Atlas (TCGA) project team was originally composed of the National Cancer Institute (NCI) and the National Human Genome Research Institute (NHGRI), and developed into A very large data research platform. Low-grade glioma (LGG) and glioblastoma (GBM), as one of the earliest tumors studied, have a large number of comprehensive data results. The LGG and GBM data in TCGA come from (https: / / genome-cancer.ucsc.edu), in which LGG included 530 tumor samples, and GBM included 167 samples. The results of CLCF1 gene transcription level are from the RNA-seq results of the Illumina HiSeq sequencing platform. figure 1 It is the expression ...

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Abstract

The invention belongs to the technical field of detection kits, and particularly relates to a kit for aided-diagnosis and prognostic evaluation on glioblastoma based on a gene CLCF1 and a use method thereof. The kit comprises a PCR (polymerase chain reaction) primer pair for amplifying the gene CLCF1 and a PCR primer pair for amplifying a housekeeping gene GAPDH. The kit has the advantages that the obvious difference of the gene CLCF1 in transcription expression levels of LGG (brain lower grade glioma) and GBM (glioblastoma multiforme) is found for the first time, the prognostic condition of apatient with the glioblastoma is well judged by the expression level of the gene CLCF1, and the gene CLCF1 can be used as the index for independently judging the prognostic condition of the glioblastoma after determining by Cox multi-factor regression analysis; after the expression condition of the transcription level of the gene CLCF1 is detected, the severity of the glioblastoma can be diagnosed, and the prognostic condition of the patient can be predicted.

Description

technical field [0001] The invention belongs to the technical field of detection kits, and in particular relates to a CLCF1 gene-based aided diagnosis and prognosis evaluation kit for glioblastoma and a use method thereof. Background technique [0002] Glioblastoma is the most malignant and most common primary brain tumor, accounting for about 50% of central nervous system tumors. Although a comprehensive program based on surgery combined with radiation and chemotherapy has become a treatment guideline, the prognosis of glioblastoma patients has not been significantly improved, and the average survival time is only 12 to 15 months. Patients with low-grade glioma (LGG) will develop into high-grade glioma (GBM) after a long or short period of time. At present, the commonly used methods to distinguish low-grade gliomas from high-grade gliomas mainly rely on histopathological diagnosis, but the results of pathological diagnosis have certain human factors and are somewhat unreli...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886
Inventor 张毅张超奇张震何谦益刘莎莎王乐宋梦佳沈志博赵启泰
Owner THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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