Kit for aided-diagnosis and prognostic evaluation on non-small cell lung cancer based on gene GALNT2 and use method thereof

A non-small cell lung cancer, auxiliary diagnosis technology, applied in the field of detection kits, can solve the problems of non-small cell lung cancer expression and clinical significance without relevant reports, and achieve a sensitive and more specific method, objective judgment, and reduced survival time. Effect

Inactive Publication Date: 2018-01-12
THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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Problems solved by technology

GALNT2 has been reported to be involved in the occurrence and development of oral squamous cell carcinoma, liver cancer and ovarian cancer, but its expression and clinical significance in non-small cell lung cancer have not yet been reported

Method used

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  • Kit for aided-diagnosis and prognostic evaluation on non-small cell lung cancer based on gene GALNT2 and use method thereof
  • Kit for aided-diagnosis and prognostic evaluation on non-small cell lung cancer based on gene GALNT2 and use method thereof
  • Kit for aided-diagnosis and prognostic evaluation on non-small cell lung cancer based on gene GALNT2 and use method thereof

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[0033] The experimental methods in the following examples are conventional methods unless otherwise specified, and the involved experimental reagents and materials are conventional biochemical reagents and materials unless otherwise specified.

[0034] 1. Comparison of the differential expression of GALNT2 in non-small cell lung cancer patients with non-small cell lung cancer and adjacent normal tissues in TCGA database

[0035] The Cancer Genome Atlas (TCGA) project team was originally composed of the National Cancer Institute (NCI) and the National Human Genome Research Institute (NHGRI), and developed into A very large data research platform. Non-small cell lung cancer samples in TCGA mainly include lung squamous cell carcinoma and lung adenocarcinoma samples. The data comes from (https: / / genome-cancer.ucsc.edu), in which 110 cases of normal adjacent tissues and 1016 cases of cancer tissues were collected. example sample. The results of GALNT2 gene transcription level are...

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Abstract

The invention belongs to the technical field of detection kits, and particularly relates to a kit for aided-diagnosis and prognostic evaluation on non-small cell lung cancer based on a gene GALNT2 anda use method thereof. The kit comprises a PCR (polymerase chain reaction) primer pair for amplifying the gene GALNT2 and a PCR primer pair for amplifying a housekeeping gene GAPDH. The kit has the advantages that the obvious difference of the gene GALNT2 in transcription expression levels of non-small cell lung cancer and normal paracancerous tissue is found for the first time, the prognostic condition of a patient with the non-small cell lung cancer is well judged by the expression level of the gene GALNT2, and the gene GALNT2 can be used as the index for independently judging the prognosticcondition of the non-small cell lung cancer after determining by Cox multi-factor regression analysis; after the expression condition of the transcription level of the gene GALNT2 is detected, the non-small cell lung cancer can be diagnosed, and the prognostic condition of the patient can be predicted.

Description

technical field [0001] The invention belongs to the technical field of detection kits, and in particular relates to a non-small cell lung cancer auxiliary diagnosis and prognosis evaluation kit based on GALNT2 gene and a use method thereof. Background technique [0002] Lung cancer is the most common tumor in the world and the greatest threat to human health and life. Non-small cell lung cancer is the type of lung cancer with the highest incidence rate. Due to the lack of typical clinical symptoms in the early stage, almost 70% of non-small cell lung cancer patients have entered the advanced stage or metastasized at the beginning of diagnosis. A global tumor data survey shows that lung cancer accounts for 17% of new tumor cases and 23% of tumor-related deaths, ranking first among tumor-related deaths. Therefore, how to find new diagnostic and prognostic factors for non-small cell lung cancer and explore its potential therapeutic value is still a hot topic in current researc...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886
Inventor 张毅张超奇李红张震董英英郑少忠李砺锋孙婷沈志博宋梦佳赵启泰
Owner THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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