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Platelet concentrate for increase of cell regeneration and cell growth

A technology for platelets and concentrates, applied in the field of degraded platelet concentrates, which can solve problems such as blood loss

Pending Publication Date: 2018-01-12
E 特尔科夫
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, there is a need to provide a sufficient amount of optimal concentration and a sufficient amount of platelet-rich plasma, since the practical method involves the loss of a considerable volume of blood

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0115] The preparation of platelet concentrates should be carried out under sterile conditions.

[0116] For further purification, the platelet concentrate can also be treated by virus inactivation.

[0117] Where synthetic or autologous platelet concentrates are used, virus removal methods may not be required.

[0118] Platelet concentrates can also be stored refrigerated for days, weeks, months or years.

[0119] Another aspect of the present invention is a novel method for preventing or treating alopecia and / or promoting hair growth using the degraded, autologously derived platelet concentrate of the present invention, said platelet concentrate containing < 50% intact platelets.

[0120] The degraded platelet concentrate can be administered as a single dose or as repeated doses.

[0121] For example, the degraded platelet concentrate of the present invention can be applied to the scalp every month, every two months, three to four months, or any other desired interval. Al...

example 1

[0182] Example 1: Freezing and Thawing Platelet Fractions to Prepare Degraded Platelet Compositions:

[0183] Platelets are frozen and thawed for cell lysis / breaking of the outer cell membrane. To freeze the platelet fraction, the solution was exposed to and kept at a temperature below -25°C for at least 2 hours without adding any cryoprotectant. Thaw at room temperature. Freeze-thaw cycles were repeated at least twice, and cell lysis was controlled by routine blood counts on a commercial cytometer. At least >80% platelets should be lysed, otherwise freeze / thaw cycles are repeated until final QC parameters reach >80% platelet lysis. The final platelet-free supernatant can be stored at a temperature below -25°C until use.

example 2

[0184] Example 2: Use of degraded platelet compositions in breast surgery

[0185] Female patients undergoing breast cancer surgery (partial mastectomy / lumpectomy) and who had planned breast reconstruction with autologous fat transfer (fat augmentation) participated in this pilot study. Before surgery, all patients were referred to the Blood Group Serum and Transfusion Unit to check eligibility for autologous PRP donation (viral load, cubital vein caliber). If eligible, a hospital visit is made and the patient is referred to the Blood Typing Serum and Transfusion Unit, where the patient is connected to a cell separator (Amicus from Fresenius Inc. TM). Within 45 minutes, 100 ml of PRP with a platelet concentration 1 / 7 that of peripheral blood was isolated, and the fraction was divided into 10 10 ml fractions, followed by freezing and storage procedures as described above. On the day of surgery, the surgeon estimated the desired volume of fat and notified the Serum and Trans...

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PUM

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Abstract

The invention refers to a decomposed platelet concentrate containing <= 50% intact platelets and optionally >= 200pg / ml RANTES and specifically to its use in the prevention or treatment of hair loss and / or for promoting hair growth. The invention further refers to a composition comprising the decomposed platelet concentrate and body fat cells and the use of said composition for reconstructive andaesthetic and curative surgery and pain relief treatment.

Description

technical field [0001] The present invention relates to degraded platelet concentrates containing ≤ 50% intact platelets and optionally ≥ 200 pg / ml chemokines, in particular for their use in preventing or treating hair loss and / or promoting hair growth. The invention also relates to a composition comprising degraded platelet concentrate and human adipocytes and said composition for use in reconstructive and cosmetic and therapeutic surgery and analgesic treatment. Background technique [0002] Whole blood is composed of various cellular components such as red blood cells, white blood cells, and platelets suspended in plasma, the body component of whole blood. Whole blood can be separated into its constituent components (cells or fluid), and one separated fraction can be administered to a patient in need of that particular fraction. [0003] Platelets, or thrombus cells, are blood cells that play a key role in wound healing and the formation of new tissue. Platelets promote...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/16A61P17/14A61L27/36A61L27/38
CPCA61K35/16A61P17/14A61K35/19A61K8/983A61K9/00A61Q5/002
Inventor E·特尔科夫M·德特克A·阿德勒
Owner E 特尔科夫