A fibrin activator, its preparation method, a kit including the activator and a detection method using the kit

Abstract

The invention discloses a fibrin activator, a preparation method of, a kit comprising the fibrin activator, and a platelet aggregative function detection method using the kit. The fibrin activator comprises the following components: a 30-100 mmol / L Tris-HCl buffer with pH of 7-8, 6-18 mg / L of batroxobin, 150-250 mg / L of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide, 20-60 g / L of PEG8000, 2-8 g / L of polylysine, 6-16 g / L of BSA, 10-50 g / L of trehalose, 0.03-0.05% of Proclin 300, 6-16mg / L of aprotinin, 10-30mg / L of cephalin and 6-12g / L of NaCl; the fibrin activator uses a protein crosslinking reagent to replace a activated coagulation facto, and the cost is low.

Description

technical field The invention relates to the technical field of platelet detection, in particular to a fibrin activator, a preparation method of the fibrin activator, a kit comprising the fibrin activator and a method for detecting platelet aggregation function using the kit. Background technique Platelets play an important role in physiological hemostasis, maintaining the integrity of blood vessel walls, and certain pathological processes, such as thrombosis, atherosclerosis, unstable angina, tumor metastasis, and inflammatory reactions. A large number of studies have shown that the activation and aggregation of platelets is the initiating factor of thrombus formation in vivo, and it is also one of the most critical components in the process of thrombus formation. Common clinical critical emergencies such as sudden cardiac death, myocardial infarction, stroke, and pulmonary infarction are all thrombotic diseases. Therefore, platelet function testing is of great significanc...

AI Technical Summary

Problems solved by technology

In order to solve the problem that the fibrin activators in the prior art all contain XIIIA activated blood coagulation factor and the reagent cost is high, the purpose of the present invention is to provide a fibrin activator

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