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Sophora flavescens regeneration system establishing technology

A technology of Sophora flavescens and system, applied in the field of establishment of Sophora flavescens regeneration system, can solve the problems of long growth cycle and low reproduction coefficient, and achieve the effect of solving the high difficulty of introduction and domestication

Inactive Publication Date: 2018-01-19
陈培党
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Sophora flavescens is mainly propagated by sowing, cutting, grafting, etc., but the reproduction coefficient is low, the growth cycle is long, and it is easily affected by external environmental conditions and other factors.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Disinfection of explants: Collect the delicate leaves of healthy plants of Sophora flavescens, gently scrub them with a soft brush dipped in washing powder water, rinse them with tap water for 1 hour, then place them in an ultra-clean workbench, first disinfect them with 75% ethanol for 10 seconds, and then use sterile Wash 4 times with water, then disinfect with 0.1% mercuric chloride solution for 6 minutes, rinse with sterile water 6 times, and then dry the water drops on the surface with sterile filter paper before use.

[0017] (2) Differentiation culture: Excise the petiole of the leaf treated in step (1), lightly scratch a few times on the leaf with a sterile scalpel, and inoculate the differentiation medium in such a way that the back of the leaf is in contact with the medium for callus Tissue and cluster bud induction. After inoculation, culture in total darkness at 26°C for 18 days, then light for 15 hours a day, the light intensity is 3100lx, and the cultu...

Embodiment 2

[0022] (1) Disinfection of explants: Collect the delicate leaves of healthy plants of Sophora flavescens, gently scrub with a soft brush dipped in washing powder water, rinse with tap water for 2 hours, and then place them in an ultra-clean workbench. Disinfect them with 75% ethanol for 8 seconds and then use sterile Wash 4 times with water, then sterilize with 0.3% mercury liter solution for 6 minutes, rinse 4 times with sterile water, and then dry the water droplets on the surface with sterile filter paper before use.

[0023] (2) Differentiation culture: Excise the petiole of the leaf treated in step (1), lightly scratch a few times on the leaf with a sterile scalpel, and inoculate the differentiation medium in such a way that the back of the leaf is in contact with the medium for callus Tissue and cluster bud induction. After inoculation, culture in total darkness at 26°C for 24 days, then light for 14 hours a day, the light intensity is 3400lx, and the culture temperature...

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PUM

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Abstract

The invention discloses a sophora flavescens regeneration system establishing technology. A sophora flavescens subshrub has a cylindrical root and a yellow scarfskin. Stems and branches are herbal shaped, green and provided with irregular longitudinal furrows. Singular pinnately compound leaves are alternate; linear stipules are formed at the lower part; leaflets have short handles, are in an ovate oval shape to a long elliptical lanceolate shape, have round or blunt point shaped tips, and round or wide wedge-shaped stem bases, and are entire. Roots can be used as a medicine, are recovered inspring and autumn, and are dried for standby use. The root of sophora flavescens is bitter in taste and cold in property, and has effects of clearing heat, eliminating dampness and killing insects. Byadopting a conventional method, the sophora flavescens has a low propagation coefficient and a long growth cycle, and is easy to be affected by factors such as external environmental conditions at the same time. While by adopting a tissue culture method, the sophora flavescens has a high propagation coefficient, material selection and propagation expansion can be carried out at an arbitrary growth stage, and convenient conditions are provided for seed selection and industrialized seedling production of sophora flavescens good stocks. According to the sophora flavescens regeneration system establishing technology provided by the invention, the sophora flavescens is adopted as an explant, a regeneration in vitro plant is obtained through the steps such as differentiation culture, multiplication culture, rooting culture, and acclimatization and transplant, and a regeneration system is established.

Description

technical field [0001] The invention belongs to the forestry category, and in particular relates to a technology for establishing a Sophora flavescens regeneration system. Background technique [0002] Sophora flavescens is also known as: bitter bone, beef ginseng, Sichuan ginseng, wild locust tree. Morphological characteristics: Subshrub. The root is cylindrical, and the outer skin is yellow. Stems herbaceous, green, with irregular longitudinal grooves. Singular pinnate compound leaves, alternate; linear stipules below; leaflets with short stalk, ovate-elliptic to long-elliptic-lanceolate, apex rounded or obtuse, base rounded or broadly cuneate, margin entire. Racemes terminal, covered with short hairs; bracts linear; flowers yellowish white; calyx bell-shaped, slightly oblique; corolla butterfly-shaped, flag petals slightly longer, apex nearly round; stamens 10, pistil 1, child The room is superior, the style is slender, and the stigma is round. Pods linear, with a lo...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 陈培党
Owner 陈培党