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Building method of rhododendron aureum leaf regeneration system

A method for establishing a method and a leaf technology, applied in the field of plant tissue culture, can solve the problems of low reproduction coefficient and long growth cycle, and achieve the effect of high reproduction coefficient and high domestication difficulty.

Inactive Publication Date: 2015-07-01
朱远星
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Rhododendron is mainly propagated by sowing, cutting, grafting, etc., but the reproduction coefficient is low, the growth cycle is long, and it is easily affected by external environmental conditions and other factors.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Disinfection of explants: Collect the delicate leaves of healthy Rhododendron spp., scrub gently with a soft brush dipped in washing powder water, rinse with tap water for 2 hours, and then place them in an ultra-clean workbench. Disinfect them with 75% ethanol for 8 seconds and then use sterile Wash 3 times with water, then sterilize with 0.1% mercury liter solution for 5 minutes, rinse 5 times with sterile water, and then dry the water drops on the surface with sterile filter paper before use.

[0019] (2) Differentiation culture: Excise the petiole of the leaf treated in step (1), lightly scratch a few times on the leaf with a sterile scalpel, and inoculate the differentiation medium in such a way that the back of the leaf is in contact with the medium for callus Tissue and cluster bud induction. After inoculation, it was first cultured in total darkness at 25°C for 20 days, then placed in light for 14 hours a day, with a light intensity of 3000lx, and placed at ...

Embodiment 2

[0024] (1) Disinfection of explants: Collect the delicate leaves of healthy Rhododendron oxderica plants, gently scrub them with a soft brush dipped in washing powder water, rinse with tap water for 3 hours, then place them in an ultra-clean workbench, first disinfect them with 75% ethanol for 10 seconds, and then use sterile Wash 5 times with water, then disinfect with 0.1% mercuric chloride solution for 8 minutes, rinse 5 times with sterile water, and then dry the water drops on the surface with sterile filter paper before use.

[0025] (2) Differentiation culture: Excise the petiole of the leaf treated in step (1), lightly scratch a few times on the leaf with a sterile scalpel, and inoculate the differentiation medium in such a way that the back of the leaf is in contact with the medium for callus Tissue and cluster bud induction. After inoculation, it was first cultured in total darkness at 27°C for 25 days, then placed in light for 15 hours a day, with an illumination int...

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Abstract

The invention discloses a building method of a rhododendron aureum leaf regeneration system. By using a conventional method, the rhododendron aureum is low in propagation coefficient, long in growth period and is easily affected by factors such as external environment conditions and the like; while a tissue culture method is adopted, the rhododendron aureum is high in propagation coefficient; material selection and propagation expansion can be carried out in any growing stage, and a convenient condition is provided for selective breeding of excellent quality and industrialized seedling production of rhododendron. According to the method, the rhododendron aureum leaf is used as an explants, the steps of differentiation culture, multification culture, rooting culture, hardening-seedling transplantation and the like are carried out to acquire a rhododendron aureum leaf regeneration in vitro plant, so that the rhododendron aureum leaf regeneration system is built; moreover, the method lays a solid foundation for industrialized seedling production and domestication culture in future, and the problem of high introduction and domestication difficulty of the rhododendron aureum is solved.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for establishing a leaf regeneration system of Rhododendron oxhide. Background technique [0002] Rhododendron is one of the top ten traditional precious flowers in China, which integrates ornamental and medicinal purposes, and is also one of the three most famous flowers in the world. my country is the center and birthplace of the distribution of rhododendrons in the world, and there are about [0003] There are nearly 600 species of flowers, accounting for about 60% of the more than 1,000 species in the world. Rhododendron is a high-quality material for landscaping because of its bright color, beautiful plant shape and early flowering period. With the increase of people's development efforts, the number of plant populations has declined sharply. Therefore, some necessary protective measures and methods should be taken to maintain th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 朱远星
Owner 朱远星