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Method for establishing tea regeneration system

A method of establishment, tea technology, applied in the field of establishment of tea regeneration system, can solve the problems of long growth cycle, low reproduction coefficient, etc., and achieve the effect of high reproduction coefficient and high difficulty in solving

Inactive Publication Date: 2019-01-29
广西玉林市华睿茶业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is mainly propagated by sowing, cutting, grafting, etc., but the reproduction coefficient is low, the growth cycle is long, and it is easily affected by external environmental conditions and other factors.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] (1) Disinfection of explants: Collect the delicate leaves of healthy tea plants, gently scrub them with a soft brush dipped in washing powder water, rinse them with tap water for 5 hours, place them in an ultra-clean workbench, disinfect them with 75% ethanol for 8 seconds, and then wash them with sterile water 7 times, then sterilized with 0.1% mercuric chloride solution for 8 minutes, rinsed with sterile water 8 times, and then dried the water droplets on the surface with sterile filter paper before use.

[0014] (2) Differentiation culture: Excise the petiole of the leaf treated in step (1), lightly scratch a few times on the leaf with a sterile scalpel, and inoculate the differentiation medium in such a way that the back of the leaf is in contact with the medium for callus Tissue and cluster bud induction. After inoculation, it was first cultured in total darkness at 26°C for 20 days, then placed in light for 12 hours a day, with a light intensity of 3000lx, and pla...

Embodiment 2

[0019] (1) Disinfection of explants: Collect the delicate leaves of healthy tea plants, gently scrub with a soft brush dipped in washing powder water, rinse with tap water for 6 hours, then place them in an ultra-clean workbench, first disinfect with 75% ethanol for 10 seconds, and then use sterile water 12 times, then sterilized with 0.1% mercuric chloride solution for 10 minutes, rinsed with sterile water for 8 times, and then dried the water droplets on the surface with sterile filter paper before use.

[0020] (2) Differentiation culture: Excise the petiole of the leaf treated in step (1), lightly scratch a few times on the leaf with a sterile scalpel, and inoculate the differentiation medium in such a way that the back of the leaf is in contact with the medium for callus Tissue and cluster bud induction. After inoculation, it was first cultured in total darkness at 28°C for 25 days, then placed in the light for 18 hours a day, with a light intensity of 3500lx, and placed ...

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PUM

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Abstract

The invention discloses a method for establishing a tea regeneration system. In a conventional method, tea leaves are low in propagation coefficient and long in growth period and are easily influencedby factors such as external environment conditions and the like. Through the adoption of a tissue culture method, the propagation coefficient is high, material selection and propagation can be carried out at any growth stage, and a convenient condition is provided for breeding and industrialized seedling culture of excellent tea varieties. According to the method provided by the invention, tea leaves are taken as an explants, tea ex-vitro regenerated plants are obtained through the steps of differentiation culture, multiplication culture, rooting culture, seedling hardening and transplanting,and the like, a tea regeneration system is established, and a solid foundation is laid for industrialized seedling culture and domestication cultivation in the future, so that the problem that introduced varieties are difficult to domesticate can be solved.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for establishing a tea regeneration system. Background technique [0002] Tea is a plant of the family Theaceae, the dried leaves of tea, mainly produced in Jiangsu, Xijiang, Jiangxi, Hubei and other places. Leaves can be picked from tea trees that have been planted for more than 3 years. It is best to pick the young buds when they have 4 to 5 leaves around Qingming. The gas is fragrant, the taste is bitter and astringent. It has the functions of clearing the head, eliminating polydipsia, reducing phlegm, eliminating indigestion, and detoxifying. Control headache, vexation and thirst, indigestion and phlegm stagnation, dysentery. With the increase of people's development efforts, the number of plant populations has dropped sharply. Therefore, some necessary protective measures and methods should be taken to maintain the quantity of wi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 陈金水肖华洲
Owner 广西玉林市华睿茶业有限公司