Molecular hybridization instrument and application method thereof

Abstract

The invention discloses a molecular hybridization instrument and an application method thereof. The molecular hybridization instrument comprises a rack body, wherein a suction head placing part, a sample placing part, a reaction part, a control manipulator and a manipulator movement driving mechanism, wherein the reaction part is provided with a rotary bin and a reaction bin, the rotary bin is rotatably arranged in the rack body, the reaction bin is arranged in a placement cavity of the rotary bin, and the reaction bin is provided with a reaction cavity; and the control manipulator is providedwith a suction head operating mechanism, a liquid adding and liquid discharging operating mechanism and a control mechanism, the control manipulator is provided with a suction head mounting station,a sample liquid sucking station as well as a sample liquid injecting station and a liquid adding and liquid discharging station which are respectively corresponding to the suction head placing part, the sample placing part and the reaction part. The molecular hybridization instrument disclosed by the invention is reasonable in structural design and smaller in volume and can be used for carrying out an experiment of detecting more samples, and time required by the experiment is shorter and experiment effect is better.

Description

technical field [0001] The invention relates to the technical field of molecular hybridization, in particular to a molecular hybridization instrument and a using method thereof. Background technique [0002] Molecular hybridization instrument, also known as: molecular hybridization box, molecular hybridization oven, is widely used in the screening of cloned genes, the production of enzyme digestion maps, the qualitative and quantitative detection of specific gene sequences in the genome, and the diagnosis of diseases. Molecular hybridization instruments are not only widely used in the field of molecular biology, but also increasingly used in clinical diagnosis. [0003] The basic principle of the molecular hybridization instrument is to use the properties of denaturation and renaturation of nucleic acid molecules to make DNA (or RNA) fragments from different sources form hybrid double-stranded molecules (heteroduplex) according to the complementary relationship of bases. Be...

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Problems solved by technology

[0004] With the wide application and promotion of molecular hybridization instruments, people have higher and higher requirements for the use of molecular hybridization instruments. Most of the existing molecular hybridization instruments can only perform low-throughput experiments, while those that can perform higher-throughput experiments Most of the molecular hybridization instruments are large in size, which occupy a lot of work space, and most of the molecular hybridization instruments used for more sample detection experiments require a long time for the experiment, which reduces people's work efficiency and is not convenient for people. work, and its experimental effect needs to be further improved

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