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Glioma prognostic marker circ7:66286511|66286709 and applications

A glioma and marker technology, applied in biochemical equipment and methods, microbiological measurement/testing, DNA/RNA fragments, etc., can solve problems such as high recurrence rate, poor prognosis, and unoptimistic postoperative survival rate

Inactive Publication Date: 2018-01-19
XIANGYA HOSPITAL CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are various treatment techniques for glioma, but due to the characteristics of fast tumor infiltration and invasion, and insensitivity to apoptosis mediated by radiotherapy and chemotherapy, the overall treatment effect is not good, the recurrence rate is high, and the prognosis is poor. Therefore, new treatments are sought. Targets and prognostic indicators are critical
At present, the diagnosis of glioma is still in the empirical stage based on clinical, pathological and imaging information, and once diagnosed, most of them are in the middle and advanced stages, and the postoperative survival rate is not optimistic

Method used

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  • Glioma prognostic marker circ7:66286511|66286709 and applications
  • Glioma prognostic marker circ7:66286511|66286709 and applications
  • Glioma prognostic marker circ7:66286511|66286709 and applications

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 Preparation of reagents for detecting the expression of circRNA circ7:66286511|66286709 for the preparation of a kit for the prognosis of glioma patients (50 reactions)

[0018] 1. RNA stabilization solution 50ml

[0019] 2. Isopropanol 100ml

[0020] 3. Chloroform 100ml

[0021] 4. Trizol 50ml

[0022] 5. Enzyme-free water 10ml

[0023] 6.1μM random reverse transcription primer 50μl

[0024] 7.5× reverse transcription buffer 200ml

[0025] 8.10mM base triphosphate deoxyribonucleotides 100μl

[0026] 9.40U / μl RNase inhibitor 500μl

[0027] 10.200U / μl MMLV reverse transcriptase 50μl

[0028] 11.Premix Ex Taq 50μl

[0029] 12.10μM circRNA circ7:66286511|66286709 real-time fluorescence quantitative PCR specific primer 30μl

[0030] circRNA circ7:66286511|66286709 Forward primer: 5'-CAGTGATTGCTCCTATGC-3',

[0031] circRNA circ7:66286511|66286709 reverse primer: 5'-CTGGCTTGATTACTTGGT-3';

[0032] 13.10 μM GAPDH specific primer 30 μl

[0033] The forward...

Embodiment 2

[0035] Example 2 Detection of tissue sample circRNA circ7:66286511|66286709

[0036] 1. Collect the glioma tissue to be tested, put it into a cryopreservation tube filled with RNA stabilization solution, and put it in a -80°C refrigerator for later use. The temperature of the refrigerator was regularly recorded by the experimenter every day.

[0037] 2. Extraction of RNA in tissues: Take an appropriate amount of specimen, add liquid nitrogen to the mortar after baking at 180°C for 6-8 hours, grind the specimen, grind to powder, add 1ml Trizol mortar specimen to the mortar, and grind into After the liquid was transferred to a tube tube, it was lysed on ice for 15 minutes. After the lysis, centrifuge at 12000rpm for 10min at 4°C, and transfer the supernatant to a new tube. Add chloroform 200μl / ml Trizol to the Tube, shake it by hand for 15-30s, place on ice for 5min, centrifuge at 12000rpm at 4°C for 15min; carefully take the upper aqueous phase into a new tube, add pre-cooled...

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Abstract

The invention discloses a glioma prognostic marker circ7:66286511|66286709 and applications, namely a reagent for detecting circRNA circ7:66286511|66286709 coming from brain tissues; and the glioma prognostic marker is used for preparing a prognostic preparation for patients with glioma. Investigators find that the expression quantity of circRNA circ7:66286511|66286709 is obviously down-regulated(P is less than 0.0001) in the brain tissues of patients with glioma, and patients with glioma having higher expression quantity of circRNA circ7:66286511|66286709 have higher postoperative survival rate (P=0.005). The prognostic prediction on the patients with glioma can be made by detecting the expression level of circRNA circ7:66286511|66286709 in glioma tissues of patients with glioma.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a circRNA marker for glioma prognosis, the application of a reagent for detecting the marker to prepare a glioma prognosis preparation, and a kit. Background technique [0002] Glioma is the most common malignant neuroepithelial cell tumor in the brain, accounting for 40.49% of intracranial tumors, and is one of the most common brain tumors in adults. From the time of diagnosis, the average life expectancy of patients with glioma is less than five years. At present, there are various treatment techniques for glioma, but due to the characteristics of fast tumor infiltration and invasion, and insensitivity to apoptosis mediated by radiotherapy and chemotherapy, the overall treatment effect is not good, the recurrence rate is high, and the prognosis is poor. Therefore, new treatments are sought. Targets and prognostic indicators are critical. At present, the diagnosis of glioma is still...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/113
Inventor 汤颖武明花李沛瑶刘庆袁健赵春花刘洋
Owner XIANGYA HOSPITAL CENT SOUTH UNIV
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