Antibacterial compounds
A compound and composition technology, applied in the field of salicylamide compounds and compositions thereof, can solve the problems of not considering the direct toxic effect of niclosamide, not considering the possible effect of drug efflux and the like
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[0269] The present invention is further described with reference to the following examples. It should be understood that the invention as claimed is not limited in any way by these examples.
[0270] Generation of E. coli strains
[0271] Disruption of PCR amplification was used by the Red recombinase method (Datsenko, K.A. and Wanner, B.L. (2000). One-stepin activation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci USA 97:6640-6645. The cassette generates deletion strains by in-frame deletion. PCR primers for disruption cassette amplification and overlap PCR are in Figure 17 described in. E. coli strain 7KO was derived from E. coli W3110 by deletion of the native nfsA, nfsB, azoR, nemA, yieF, ycaK and mdaB genes. E. coli strain 7KOΔtolC was derived from 7KO by deletion of the native tolC gene. The E. coli strain 7KOΔtolC (DE3) with an integrated λDE3 prophage allowing expression via induction of T7 RNA polymerase was derived from 7K...
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