Method and Application of Detecting Insect Lifespan Using Heat Shock Protein hsp70 Gene
A heat shock protein and lifespan technology, which is applied in application, genetic engineering, plant genetic improvement, etc., can solve the problems of time-consuming, labor-intensive, high economic investment, etc., and achieve the effect of simple and easy detection method
Active Publication Date: 2020-04-07
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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Problems solved by technology
At present, the more traditional method to evaluate the lifespan of adult lacewing products fed by different foods or from different business sources is to count the time from the day of eclosion to natural death, which is time-consuming, laborious, and costly. Therefore, it is urgent to find a fast and An economical method to detect important biological characteristics of natural enemy insect commodities - lifespan method
Method used
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Embodiment 1
[0052]Example 1: Identification of insect heat shock protein Hsp70 coding gene and design of special primers for qRT-PCR
[0053] Studies have shown that the heat shock protein Hsp70 is closely related to the aging process and has a positive effect on prolonging the lifespan of insects. It is a good molecular marker for detecting insects' lifespan. Therefore, the present invention uses the expression level of the heat shock protein Hsp70 gene of the lacewing to detect and evaluate its lifespan.
[0054] The nucleotide sequence of the heat shock protein Hsp70 of the lacewing is sequence 1 in the sequence listing, and the amino acid sequence encoded by it is sequence 2 in the sequence listing.
[0055] The special primers designed for qRT-PCR amplification of the heat shock protein Hsp70 gene of the greater lacewing are as follows:
[0056] Upstream primer: 5'-CTGCAGAAGCGTATTTAGGCC-3' (SEQ ID NO: 3)
[0057] Downstream primer: 5'-GCTAATGCTGCTGCTGTCGG-3' (SEQ ID NO: 4)
Embodiment 2
[0058] Embodiment 2: the application of heat shock protein Hsp70 (Heat shock protein 70 (HSP70)) gene in detecting the lifespan of lacewing
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The invention provides a method for detecting lifetime of insects by utilizing a heat shock protein Hsp70 gene and application of detection of an expression quantity of a heat shock protein Hsp70 coding gene to detection of the lifetime of the insects. The nucleotide sequence of the heat shock protein Hsp70 coding gene is a sequence 1 in a sequence table. The method provided by the invention has the beneficial effects that a test proves that the detection method provided by the invention can be used for detecting and estimating the lifetime of the insects of different population through detecting the expression quantity of the heat shock protein Hsp70 gene; the detection method is mainly used for detecting the lifetime of chrysopa septempunctata adults with different food nutrient sources.The test proves that the detection method provided by the invention can detect the lifetime of the insects by only 2 to 3 days; however, a traditional biological method needs 30 to 60 days. The detection method provided by the invention has the advantages of simplicity and feasibility, easiness of obtaining materials and convenience for operation, and is an extremely economical and practical method suitable for inspecting and detecting commercialized insect products.
Description
technical field [0001] The invention relates to the field of biotechnology, and more specifically relates to a method and application for detecting lifespan of insects by using heat shock protein Hsp70 gene. Background technique [0002] Pest biological control plays an important role in the sustainable development of agriculture and forestry and green plant protection. Lacewing is a very good natural enemy resource. It has a significant control effect on the growth and decline of various pest populations in nature. It mainly preys on aphids, whiteflies, mites, small Lepidoptera larvae, thrips, scale insects and Liriomyza larvae, etc., have broad and far-reaching application prospects. It is an important condition for its successful application in biological control to obtain high-quality large lacewings through mass reproduction. At present, the commercial production of many kinds of lacewings has been realized in the world, and the maximization of profits by reducing pro...
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IPC IPC(8): C12Q1/6888C12Q1/686C07K14/435C12N15/12
Inventor 王孟卿王娟刘晨曦张礼生郭义唐艺婷陈红印
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI