Microfluidics chip for detecting mixing with prestored liquid after quantitative flow separation

Abstract

The invention provides a microfluidics chip for detecting mixing with prestored liquid after quantitative flow separation. The microfluidics chip comprises an upper cover sheet, a middle elastic thinfilm and a lower base sheet, wherein the upper cover sheet comprises a pneumatic micro-valve control passage; the lower base sheet comprises a liquid inlet, a liquid outlet, an air inlet of the liquidpassage, a liquid storage tank, a waste liquid tank, a liquid passage, an impedance sensor and an absorbancy testing passage; an open end of the control passage is connected with a pipeline, the pipeline is connected with an electromagnetic valve, the electromagnetic valve is connected with a vacuum system and a pressure system, a PDMS (polydimethylsiloxane) thin film under the passage is controlled to protrude up or press down the liquid passage, and the switching is controlled by software; the air inlet of the liquid passage is connected with an air steel cylinder or an injector; the liquidinlet is connected with a pipeline, and the pipeline is connected with an injection pump; a dissolving agent and thinning liquid are respectively presetored into the two liquid storage tanks. The microfluidics chip has the advantages that the large-ratio sample mixing is realized, the result is accurate and reliable, and a new technology is provided for the quantitative flow separating of the micro-fluid solution.

Description

technical field [0001] The invention relates to a chip structure, in particular to a microfluidic chip for quantitative splitting and mixing with pre-existing liquid for detection. Background technique [0002] As a new type of test device, microfluidic chip has the advantages of small consumption, large throughput, high efficiency and low cost, and has begun to be used in chemical analysis, biological detection and environmental monitoring and other fields. In the microfluidic analysis chip, how to realize the quantitative shunting of microfluid has always been a research problem and hot spot in the microfluidic analysis chip technology in recent years. Quantitative injection of microfluidics is the key to sample processing and analysis, such as in biological assays, phase change reactions, and multicomponent reactions. Some of these methods can realize quantitative shunting, such as electrical comparison method, entrainment sampling method based on cross channel, etc. Ho...

AI Technical Summary

Problems solved by technology

However, the electrical analogy method can only be used as an approximate solution method, and cannot accurately design a high-precision fluid network; the cross-channel-based entrainment sampling method needs to add a buffer in the channel, which affects the concentration of the sample, and the composition of the solution Different ion components with different mobilities have injection discrimination effect, which makes the method subject to certain limitations

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