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A Composition For Repairing Chondral Defects

A technology of composition and cartilage, which is applied in the direction of drug combination, bone diseases, medical preparations containing active ingredients, etc., and can solve problems such as increasing the burden on surgeons

Active Publication Date: 2018-02-16
BIOTEGY CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This may increase the surgeon's burden and cause several problems, such as periosteal hypertrophy (Kreuzetal., Osteoarthritis Cartilage.2007Dec; 15(12):1339-47; Henderson I et al., Arthroscopy.2006Dec; 22(12):1318 -1324)

Method used

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  • A Composition For Repairing Chondral Defects
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  • A Composition For Repairing Chondral Defects

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1. In vitro studies

[0049] 1. Preparation of Bone Marrow Concentrate (BMC)

[0050] Porcine bone marrow concentrates were rapidly isolated using a sucrose gradient. Briefly, pig bone marrow was withdrawn from the tibia via a syringe. The bone marrow was then filtered through a 70 micron mesh. Filtered bone marrow (10 mL) was added to a 17.5% sucrose gradient (15 mL) in a 50 mL conical tube and centrifuged once at 1,500 rpm for 5 minutes. The bone marrow concentrate in the fractionated layer was then extracted by centrifugation at 1,500 rpm for 5-10 minutes. The obtained pellet was dispersed into 1 ml of DPBS to count the total cell number by an automatic cell counter.

[0051] 2. Preparation of Bone Marrow Mesenchymal Stem Cells (BM-MSCs)

[0052] The bone marrow concentrate collected from the fractionated layer was transferred to a new tube and centrifuged at 1,500 rpm for 5-10 minutes to obtain nucleated cells. These cells were seeded into culture flas...

Embodiment 2

[0068] Example 2. Animal studies

[0069] 1. Preparation of Bone Marrow Concentrate (BMC)

[0070] Porcine bone marrow concentrates were rapidly isolated using a sucrose gradient. Briefly, 10 ml of porcine bone marrow was aspirated from the tibia via syringe. Filtered bone marrow (10 mL) was added to a 17.5% sucrose gradient (15 mL) in a 50 mL conical tube and centrifuged once at 1,500 rpm for 5 minutes. The bone marrow concentrate in the fractionated layer was then extracted by centrifugation at 1,500 rpm for 5-10 minutes. The pellet was dispersed into 1 ml of DPBS to count the total cell number by an automated cell counter.

[0071] 2. Preparation of Cartilage Tissue Fragments

[0072]Cartilage tissues were obtained from pig knee joints and washed with DPBS solution. Mince the tissue into small pieces with a scalpel. Cartilage fragments approximately 1-2 mm x 2-3 mm in size were washed with DPBS and collected by centrifugation at 1,500 rpm for 5-10 minutes.

[0073] 3...

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Abstract

A composition for repairing chondral defects is provided. The composition comprises a bone marrow concentrate, a cartilage fragment or powder, and a synovial membrane. Also provided is a method for treating a chondral defect in a subject. Said method comprises a step of applying a composition of the present invention into the chondral defect, and applying a fibrin glue onto the composition to cover the chondral defect.

Description

technical field [0001] The present invention relates to a composition for repairing cartilage defects. Background technique [0002] Tissue engineering using autologous chondrocytes has gradually become a promising method for cartilage regeneration. However, many concerns remain regarding the use of autologous chondrocytes, such as injury at the harvest site and their inherent propensity to lose their phenotype during monolayer expansion. Mesenchymal stem cells (MSCs) are considered the cell type of choice because of their ability to differentiate into chondrocytes in a single-step program without the need for a biopsy / culture period and staged reimplantation surgery (Fang HW , Biomed Eng 21:149–155 (2009)). A key to successful cartilage regeneration from MSCs is the correct modulation of the cells to differentiate into the chondrogenic pathway and express normal cartilage extracellular matrix (ECM). In addition, the ECM has been identified as being significantly involved...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/32A61K35/28A61L27/38A61P19/08A61P19/02
CPCA61K35/32A61L27/3608A61L27/3612A61L27/3654A61L27/38A61P19/02A61P19/08
Inventor 方旭伟张至宏陈佳君
Owner BIOTEGY CORP
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