Autologous living cell soft tissue filling gel and preparation method thereof
A soft tissue and living cell technology, applied in the field of human soft tissue fillers, can solve problems such as imperfect fibroblasts, and achieve the effects of high synthetic function and high survival rate
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[0015] The autologous living cell soft tissue filling gel of the present invention, including culture and expansion to at least 8×10 8 Individual fibroblasts, platelet-rich plasma and thrombin-calcium with a platelet concentration that is more than 8 times the normal blood concentration.
[0016] The thrombin-calcium preparation is prepared by dissolving 1000U thrombin in 1ml 10% calcium chloride, which belongs to the conventional preparation method.
[0017] The concrete preparation steps of filling gel of the present invention are:
[0018] In the first step, add 20ml of autologous peripheral venous blood into two 10ml blood collection tubes filled with serum separation gel and anticoagulant, centrifuge at 315×g centrifugal force for 10min with a desktop low-speed self-balancing centrifuge, and use a 10ml syringe to extract serum for separation Add all the plasma above the gel into two ordinary 10ml blood collection tubes, centrifuge at 260×g for 5 minutes, and extract 1ml ...
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