Autologous living cell soft tissue filling gel and preparation method thereof

A soft tissue and living cell technology, applied in the field of human soft tissue fillers, can solve problems such as imperfect fibroblasts, and achieve the effects of high synthetic function and high survival rate

Inactive Publication Date: 2012-06-13
侯强 +4
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a more reliable and complete upgraded product, that is, autologous living cell soft tissue filling gel, in order to solve the imperfect problem of the previous autologous skin fibroblast transplantation.

Method used

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Embodiment Construction

[0015] The autologous living cell soft tissue filling gel of the present invention, including culture and expansion to at least 8×10 8 Individual fibroblasts, platelet-rich plasma and thrombin-calcium with a platelet concentration that is more than 8 times the normal blood concentration.

[0016] The thrombin-calcium preparation is prepared by dissolving 1000U thrombin in 1ml 10% calcium chloride, which belongs to the conventional preparation method.

[0017] The concrete preparation steps of filling gel of the present invention are:

[0018] In the first step, add 20ml of autologous peripheral venous blood into two 10ml blood collection tubes filled with serum separation gel and anticoagulant, centrifuge at 315×g centrifugal force for 10min with a desktop low-speed self-balancing centrifuge, and use a 10ml syringe to extract serum for separation Add all the plasma above the gel into two ordinary 10ml blood collection tubes, centrifuge at 260×g for 5 minutes, and extract 1ml ...

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Abstract

The invention discloses autologous living cell soft tissue filling gel, which comprises autologous fibroblasts cultivated and amplified to 8*108, platelet-rich plasma of which the platelet concentration is 8 times that of normal blood concentration and a thrombin-calcium agent. The preparation method comprises the following steps of: amplifying the extracted fibroblasts to 8*108 in vitro; adding into the prepared platelet-rich plasma; activating with the thrombin-calcium agent; and coagulating fibrinogens in the plasma into fibrin glue with a netlike structure to obtain the living cell soft tissue filling gel, wherein culture media used in primary passage and passage of fibroblasts are prepared from a serum-free culture medium and 5 percent of platelet-rich plasma. The preparation method has the advantages that: the problems of in-vitro amplification and culturing of fibroblasts, collection and feedback, keeping of a cell healthy state in an in-vivo survival multiplication process, survival after feedback, nutrition supply of cell multiplication, space release of cell multiplication, and the like are solved, and high survival rate of cells is ensured.

Description

technical field [0001] The invention relates to a soft tissue filler for human body prepared by utilizing biological tissue engineering technology and the principle of cell regenerative medicine, in particular to an autologous living cell soft tissue filling gel, and also relates to a preparation method of the filling gel. Background technique [0002] In recent years, with the high prosperity of the medical beauty market, people's pursuit of beauty has gradually shifted from the initial stage to a higher level of natural and healthy beauty, which also puts forward higher requirements for the beauty technology of medical beauty institutions. Require. In the current cosmetic surgery, in addition to the conventional orthopedic cosmetic surgery, micro-plastic facial soft tissue filling accounts for more than 30% of the share. Taking hyaluronic acid filling as an example, the annual output value is as high as more than 7 billion. The reason why hyaluronic acid can achieve such...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/36A61L27/22A61L27/02A61L27/52A61L27/54
Inventor 侯强秦军侠潘万立郭栋梁吴刚
Owner 侯强
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