Method for preparing modified soybean protein and pectin composite film
A soy protein and composite film technology, which is applied in the field of natural polymer film materials and photosensitive, can solve problems such as toxicity and destroy the biocompatibility of soy protein, achieve low cost, good promotion and application prospects, and avoid chemical cross-linking agents. effect used
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Embodiment 1
[0017] The preparation of embodiment 1 photosensitive monomer VM
[0018] Dissolve 37.0g of 7-hydroxy-4-methylcoumarin, 14.14g of KOH, 2g of polyvinyl alcohol (PEG2000), and an appropriate amount of potassium iodide in 100mL of deionized water, and heat to 40°C with stirring. After 10 minutes, 30.4 g of p-chloromethylene styrene (treated by basic alumina column chromatography) was added, and reacted at 40° C. for 48 hours. After the system was cooled, the product precipitated in water to obtain a milky yellow solid. The product was dissolved in tetrahydrofuran, precipitated in a 3:1 mixture of methanol and water, and repeated three times to obtain a white solid. Finally, the purified product was vacuum-dried at 40°C for 12 hours to obtain the photosensitive monomer VM.
Embodiment 2
[0019] The preparation of the urea buffer solution of embodiment 2pH≈8.5
[0020] Use 8mol / L urea solution to prepare 0.1mol / L NaOH solution, 0.4mol / L boric acid solution and 0.4mol / L KCl solution respectively, then pipette 10.1ml NaOH solution, 12.5ml boric acid solution and 12.5ml KCl solution respectively Put the solution in a 100mL volumetric flask, and dilute to volume with 8mol / L urea solution to obtain a urea buffer solution with pH ≈ 8.5.
Embodiment 3
[0021] The preparation of embodiment 3 modified soybean protein
[0022] Add 3g soybean protein isolate and 100mL 8mol / L urea buffer solution with pH ≈ 8.5 to the three-neck flask, stir at 60°C for 40min under nitrogen, then add 300uL mercaptoethanol, react for 1h, and then add 50mL Composite initiator (0.1mol / L) and 3gVM monomer reacted at 60°C for 4h and then stopped the reaction. Pour the reaction product into 1L acetone for sufficient precipitation, centrifuge, extract the obtained precipitate with THF in a Soxhlet extractor for 24 hours to remove unreacted monomers and homopolymers, and dry the obtained product in a vacuum Dry to constant weight in a container to obtain the modified product SPI-VM. Composite initiators are potassium persulfate and ammonium cerium nitrate.
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