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Rapid sample-injection method for flow-type analysis instrument

A technology of flow analysis and sample injection, which is applied in the direction of instruments and analytical materials, etc., can solve the problems of irrelevance of sample injection flux, and achieve the effect of improving detection throughput, shortening waiting time for sample injection, and improving sample detection throughput

Active Publication Date: 2018-02-23
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is a patent to improve the sampling method (CN101680823), but the point of the invention lies in the control of the sampling residue, which has nothing to do with improving the sampling throughput

Method used

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  • Rapid sample-injection method for flow-type analysis instrument
  • Rapid sample-injection method for flow-type analysis instrument
  • Rapid sample-injection method for flow-type analysis instrument

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Embodiment Construction

[0027] The present invention will be further described in detail below in conjunction with the accompanying drawings, so that those skilled in the art can implement it with reference to the description.

[0028] figure 1 An implementation form of a liquid flow system of a flow analysis instrument is shown, figure 2 A control schematic diagram of the flow analysis instrument injection process is shown. refer to figure 1 , the liquid flow system diagram of the flow analysis instrument includes a sheath liquid barrel 1, a filter element 2, a sheath liquid pump 3, a sample tube 4, a sample pump 5, a flow cell 6 and a waste liquid barrel 7, and the sample placed in the sample tube 4 is The sample pump 5 enters the flow cell 6 , and the sheath fluid from the sheath fluid bucket 1 passes through the detection area and then is discharged into the waste fluid bucket 7 . refer to figure 2 , the sample is excited in the flow chamber and the signal is received by the detector 8 and ...

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Abstract

The invention discloses a rapid sample-injection method for a flow-type analysis instrument. The rapid sample-injection method comprises the following steps: presetting an event time ratio firstly under a sample-injection speed needed for detection; when the sample injection is started, loading a sample at a high speed, adopting a detection module of the instrument to judge the sample injection stage, and when the even time ratio can be obtained for a plurality of times by fewer-event detection, judging that a part of the sample reaches a detection area; then reducing the sample-injection speed to a normal detection sample-injection speed in a step-by-step manner, carrying out real-time judgment in the process, after the preset event time ratio is obtained again at the normal detection sample-injection speed, carrying out multiple fewer-event sampling at an equal interval, and if the preset event time ratio can be obtained respectively, showing that the sample is stably injected and starts to pass through the detection area normally and the system can conduct actual detection and data acquisition. The rapid sample-injection method disclosed by the invention has the advantages thatthe sample-injection waiting time of the analysis instrument can be obviously reduced, the labor cost can be saved and the detection flux of the instrument can be increased.

Description

technical field [0001] The invention relates to a sampling method for a flow analysis instrument. More specifically, the present invention relates to a rapid sample injection method for a flow analysis instrument. Background technique [0002] Flow analysis instruments include flow cytometers, liquid phase suspension chip decoding analyzers, urine sediment analyzers and other commonly used instruments in clinical diagnosis and scientific research. The detection principles of these instruments are all based on flow focusing technology: under the action of high-speed sheath fluid, the samples are focused and passed through the detection area one by one. In order to ensure the accuracy of sample testing, slow sample injection is usually required. Due to the presence of necessary modules such as pump valves and pipelines in the sample flow path, a certain amount of waiting time is required between sample loading and actual detection, which reduces the throughput of these instr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N35/10
CPCG01N35/1095
Inventor 裴智果钟金凤王策马玉婷严心涛武晓东
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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