Unlock instant, AI-driven research and patent intelligence for your innovation.

A preparation for preventing and treating postharvest gray mold disease of fruit

A technology for botrytis and fruit, applied in the field of preparations for the prevention and treatment of postharvest botrytis, can solve the problems of soil texture change, reduction of control effect, crop residue, etc., and achieve high-efficiency control effect

Active Publication Date: 2019-08-20
INST OF BOTANY CHINESE ACAD OF SCI
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the long-term use of chemical agents has continuously enhanced the resistance of Botrytis cinerea, resulting in a greatly reduced control effect; in addition, the above-mentioned types of fungicides are highly toxic agents, which are very easy to remain in crops in large quantities, resulting in toxic residues in fruits and vegetables. The substance exceeds the standard, and long-term consumption will cause great harm to the human body; on the other hand, a large amount of chemicals accumulate in the soil, resulting in changes in soil texture and bringing huge harmful pressure to the ecological environment

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A preparation for preventing and treating postharvest gray mold disease of fruit
  • A preparation for preventing and treating postharvest gray mold disease of fruit
  • A preparation for preventing and treating postharvest gray mold disease of fruit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Use an inoculation needle to cut 1mm on the PDA plate with Botrytis cinerea 2 The culture block with hyphae was inoculated into a PDA plate with a diameter of 90mm, and cultured in a 25°C incubator. The concentrations of methyl limonate in the PDA medium were 60, 120 and 180 mg / L respectively.

[0028] Measure bacterium colony diameter every 12 hours afterwards, measure altogether 4 days, measure 6 plates for each treatment concentration, the result is as follows figure 1 As shown, it can be seen that methyl limonate has significant inhibitory effect on Botrytis cinerea, and wherein the concentration of methyl limonate in the medium is the best inhibitory effect of 180mg / L.

Embodiment 2

[0029] Embodiment 2, the inhibitory effect of methyl limonate on botrytis cinerea after apple stab wound inoculation

[0030] Select healthy apple fruits with coloring and uniform size, soak them in 2wt% sodium hypochlorite solution for 2 minutes, wash them with clean water and dry them naturally.

[0031] The inoculation needle was burnt on the flame of an alcohol lamp for 1 min, and after natural cooling, it was pierced at the equator in the center of the fruit, with a hole depth of 3 mm and a diameter of 3 mm.

[0032] Add 5 μL of Botrytis cinerea spore suspension to the punctured hole at a concentration of 1×10 5 spores / mL and air-dried for 1 h.

[0033] Add 10 μL of methyl limonate aqueous solution to the punctured hole, the concentrations are 600, 3000 and 6000 mg / L, respectively, and add 10 μL of sterile water to the control group.

[0034] Add absorbent paper soaked with distilled water to moisturize, seal with a fresh-keeping bag, observe and measure the incidence o...

Embodiment 3

[0035] Embodiment 3, the inhibitory effect of methyl limonate on botrytis cinerea after pear stab wound inoculation

[0036] Select healthy pear fruits that are colored and uniform in size, soak them in 2% sodium hypochlorite solution for 2 minutes, wash them with clean water, and then dry them naturally.

[0037] The inoculation needle was burnt on the flame of an alcohol lamp for 1 min, and after natural cooling, it was pierced at the equator in the center of the fruit, with a hole depth of 3 mm and a diameter of 3 mm.

[0038] Add 5 μL of Botrytis cinerea spore suspension to the punctured hole at a concentration of 1×10 5 spores / mL and air-dried for 1 h.

[0039] Add 10 μL of methyl limonate aqueous solution to the punctured hole, the concentrations are 600, 3000 and 6000 mg / L, respectively, and add 10 μL of sterile water to the control group.

[0040] Add absorbent paper soaked with distilled water to moisturize, seal with a fresh-keeping bag, observe and measure the inc...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation for preventing and treating gray mold after fruit picking. The preparation is an aqueous solution of methyl thujate, and the mass volume concentration is 0-6,000mg / L but is not zero. A method for preventing and treating the gray mold after fruit picking provided by the invention comprises the following steps: inoculating the botrytis cinerea infected fruits with the aqueous solution of methyl thujate to realize prevention and control of the gray mold after fruit picking, wherein the mass volume concentration of the aqueous solution of methyl thujate is 0-6,000mg / L but is not zero; the inoculation amount of the aqueous solution of methyl thujate is 5-10mu L. According to the preparation disclosed by the invention, through systematic research on the disease occurrence law after fruit picking, an inhibitory effect of the methyl thujate is discovered that the methyl thujate has an efficient prevention and control effect on the occurrence and progression of fruit diseases. The preparation for preventing and treating the gray mold after fruit picking disclosed by the invention, namely the methyl thujate, can prevent and control the botrytis cinerea infected fruits, and realizes an efficient prevention and control effect on the occurrence and progression of fruit diseases.

Description

technical field [0001] The invention relates to a preparation for preventing and treating postharvest gray mold disease, and belongs to the field of biotechnology. Background technique [0002] Fruit is an important part of human dietary structure. Because of its high sugar content and easy damage after harvest, it is infected by pathogens and causes fruit to rot. Therefore, the prevention and control of postharvest pathogen infection is a key issue in fruit storage and preservation. Botrytis cinerea is caused by Botrytis cinerea (also known as gray mold) in the Ascomycota subphylum Botrytis, which is a common and difficult to control fungal disease; Botrytis cinerea is harmful to crop stems, Leaves, flowers, and fruits can all cause harm, especially to fruits, affecting fruit yield and quality, and causing great economic losses. [0003] Chemical control is the most widely used method to control Botrytis cinerea. Its killing effect is remarkable and the operation is simple...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01N37/08A01N25/02A01P3/00
Inventor 田世平陈彤秦国政徐勇
Owner INST OF BOTANY CHINESE ACAD OF SCI