Method for differentiating ureaplasma urealyticum and ureaplasma parvum in ureaplasma with culture method

Abstract

The invention discloses a method for differentiating ureaplasma urealyticum and ureaplasma parvum in ureaplasma with a culture method. The method comprises the following steps: determining two detection micropores which are respectively marked as a first hole and a second hole, wherein the first hole does not contain additive, a manganese and magnesium solution is added into the second hole, and magnesium and manganese ions are attached to the inner wall of the second hole after being dried; adding a sample to be tested into a ureaplasma culture medium, after even mixing, respectively adding into the first hole and the second hole, and culturing mixture for 24-48 hours at 37 DEG C; according to the color change situation of the first hole and the second hole, determining whether the ureaplasma urealyticum and the ureaplasma parvum are contained in a sample or not. The preparation of a kit through the method disclosed by the invention is more convenient and practical. The authenticationmethod is simple in operation, whether a sample contains the ureaplasma urealyticum and the ureaplasma parvum or not can be accurately differentiated through one step, meanwhile, the method can be combined with drug sensitivity detection, and meanwhile, an authentication and drug allergy result can be carried out. The detection kit prepared according to the method principle disclosed by the invention has the advantages of visual and accurate authentication result and is convenient for clinical popularization and use.

Description

technical field [0001] The invention relates to biological detection technology, in particular to a method for distinguishing Ureaplasma urealyticum and Ureaplasma micronum in Ureaplasma by a culture method. Background technique [0002] Ureaplasma (Ureaplasma) was first isolated from human reproductive tract specimens as a T strain in 1954, and in 1974, it was named as a new species - Ureaplasma urealyticum (Ureaplasma urealyticum) by Shepard MC et al. Ureaplasma urealyticum is divided into two biological groups: Parvo group and T960 group, which can be further divided into 14 serotypes, Parvo group consists of serotypes 1, 2, 6, and 14, and T960 group includes 2, 4, There are ten serotypes including 5, 7, 8, 9, 10, 11, 12, and 13. The appearance of the colonies formed by the culture of these two groups is consistent, and the division of biogroups can only be based on the differences between the genomes, which requires the use of nucleic acid detection methods. It is also...

AI Technical Summary

Problems solved by technology

Single-primer PCR cannot distinguish between UU and UP, and what is detected is UU and UP; multi-primer multiplex PCR and gene sequencing can distinguish UU and UP, but the operation is too complicated and not suitable for clinical use, and drug sensitivity testing cannot be performed at the same time

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