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Microbial detoxification method of cottonseed cakes

A technology of cottonseed cake and microorganisms, which is applied in the field of microbial treatment of cottonseed cakes, and can solve the problems of complicated process, unsuitable for small-scale enterprise production and application, and high cost

Inactive Publication Date: 2018-03-06
黄增才
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Existing detoxification methods are costly and complicated, and are not suitable for small-scale enterprise production and application

Method used

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  • Microbial detoxification method of cottonseed cakes

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Embodiment Construction

[0014] Both Aspergillus niger and Saccharomyces cerevisiae used in the present invention are commercially available.

[0015] Aspergillus niger was activated on the slant medium, and then inserted into the seed medium for 30°C constant temperature shaker culture, and after 48 hours, it was used as a solid-state fermentation seed liquid. Saccharomyces cerevisiae was activated on the slant medium, and then inserted into the seed medium to cultivate in a constant temperature shaker at 30°C. After 48 hours, it was used as a solid-state fermentation seed liquid. The components of the slant medium are: 40g potato, 4g glucose, 4g agar and 200g water; the pH is natural, 0.1 MPa, and sterilized at 121°C for 30 min. The seed culture medium is calculated by weight percentage: 40g of potatoes, 4g of glucose and 200g of water; the pH is natural, 0.1 MPa, and sterilized at 121°C for 30 minutes.

[0016] The mixed bacterial solution of Aspergillus niger and Saccharomyces cerevisiae was inoc...

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Abstract

The invention discloses a microbial detoxification method of cottonseed cakes. A cottonseed cake solid fermentation medium is inoculated with a mixed bacterium liquid containing aspergillus niger andsaccharomyces cerevisiae for solid fermentation, wherein a fermentation temperature is 30 DEG C, and inoculation amount V / W is 15%, and initial moisture content is 48% in percentage by weight, and initial pH value is 6, and fermentation time is 60 h. The detoxification rate of free gossypol in fermented cottonseed cakes is 95.51%, and true protein content is increased from 32.99% to 42.40%, and lysine, methionine and threonine in essential amino acids separately increase by 31.03%, 25.00% and 44.23% compared with those before fermentation, and NSI (nitrogen soluble index) increases by 1.49 times, so that the quality of the cottonseed cakes is improved

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to a method for treating cottonseed cake with microorganisms. Background technique [0002] my country is one of the largest cotton producing countries in the world, with about 10 million tons of cottonseed per year, and the amount of resources ranks first in the world. Cottonseed cake is one of the most important vegetable protein resources, and it is a by-product after extracting cottonseed oil. The annual output of cottonseed cake in my country is about 6 million tons. After hydrolysis of cottonseed cake in my country, 17 kinds of amino acids can be obtained, among which essential amino acids are relatively close to soybean protein. [0003] Although cottonseed meal is rich in nutrition, gossypol toxin, which accounts for 0.7%-4.8% of ordinary cottonseed and 0.2-2.8% of cottonseed meal, restricts its utilization space. [0004] Gossypol is also called gossypol toxin. The p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L5/20
Inventor 黄增才
Owner 黄增才