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Method for extracting nutrient solution from marine wastes

A waste and nutrient solution technology, applied in food science, application, food ingredients, etc., can solve the problems of high and low histamine content, achieve the effects of protecting the environment, avoiding decay, and strong operability

Inactive Publication Date: 2018-03-06
荣成市日鑫水产有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the deficiency of high histamine content in existing marine waste products, the object of the present invention is to provide a method for extracting nutrient solution from marine waste with reasonable process, advanced technology and strong operability. The nutrient solution is rich in nutrients such as polypeptides, free amino acids, and soluble proteins, and has low histamine content

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for extracting nutrient solution from marine waste, which goes through the following steps:

[0026] (I) Pretreatment of animal-based seafood waste: remove the shellfish shell and the obviously decaying part of the seafood waste, and crush the seafood waste into 5-10 mm size particles, and mix with 1 times the weight of water ;

[0027] (II) Add 55 parts by weight of the seafood waste of step I to 4500 U / mL with trypsin, and enzymolyze it at 42 degrees Celsius for 16 hours, and heat it at 85 degrees Celsius for 15 minutes for inactivation;

[0028] (III) 120 parts by weight of the seafood waste of step I was added with alkaline protease to 2400 U / mL, and enzymatically hydrolyzed at 42 degrees Celsius for 16 hours, and heated at 85 degrees Celsius for 15 minutes for inactivation;

[0029] (IV) Mix the enzymatic hydrolysis mixture of steps II and III, and follow the steps of 2.0×10 6 The amount of CFU / mL is inoculated with Omer Kodak yeast, fermented at 42 degrees Celsi...

Embodiment 2

[0032] A method for extracting nutrient solution from marine waste, which goes through the following steps:

[0033] (I) Pretreatment of animal seafood waste: remove the shellfish shell and the obviously decaying part of the seafood waste, and crush the seafood waste into 5-10 mm particles, and mix with 1.5 times the weight of water ;

[0034] (II) Add 50 parts by weight of the seafood waste of step I to 3000 U / mL with trypsin, and enzymolyze it at 45 degrees Celsius for 18 hours, and heat it at 85 degrees Celsius for 30 minutes for inactivation;

[0035] (III) Add 100 parts by weight of the seafood waste of step I to 4000 U / mL with alkaline protease, and enzymolyze it at 40 degrees Celsius for 12 hours, and heat it at 95 degrees Celsius for 15 minutes for inactivation;

[0036] (IV) Mix the enzymatic hydrolysis mixture of steps II and III, and follow the steps of 1.50×10 6 The amount of CFU / mL is inoculated with Omer Kodak yeast, fermented at 40 degrees Celsius for 18 hours, and hea...

Embodiment 3

[0039] A method for extracting nutrient solution from marine waste, which goes through the following steps:

[0040] (I) Pretreatment of animal seafood waste: remove the shellfish shells and the obviously decaying part of the seafood waste, and crush the seafood waste into 5-10 mm size particles, and mix with 1.2 times the weight of water ;

[0041] (II) 70 parts by weight of the seafood waste of step I was added with trypsin to 6000 U / mL, and enzymatically digested at 40 degrees Celsius for 12 hours, and heated at 95 degrees Celsius for 15 minutes for inactivation;

[0042] (III) 110 parts by weight of the seafood waste of step I was added with alkaline protease to 1400 U / mL, and enzymatically hydrolyzed at 45 degrees Celsius for 18 hours, and heated at 95 degrees Celsius for 30 minutes for inactivation;

[0043] (IV) Mix the enzymatic hydrolysis mixture of steps II and III, and follow the steps of 3.0×10 6 The amount of CFU / mL was inoculated with Omer Kodak yeast, fermented at 45 d...

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Abstract

The invention discloses a method for extracting a nutrient solution from marine wastes. The method comprises the following steps: (I) pretreating marine wastes, namely removing obviously decayed partsin shellfish shells and marine wastes, and grinding the marine wastes into particles with the size of 5-10mm; (II) adding trypsin into 50-70 weight parts of the marine wastes in the step I, and performing inactivation after enzymolysis; (III) adding alkaline protease into 100-120 weight parts of the marine wastes in the step I, and performing inactivation after enzymolysis; (IV) mixing the enzymolysis mixture of the steps II and III, inoculating Kodamaea ohmer, and performing inactivation after fermentation; and (V) filtering the fermentation mixture obtained in the step IV, thereby obtainingthe nutrient solution. According to the method disclosed by the invention, the prepared extract is enriched in nutrient substances such as polypeptides, free amino acids and soluble proteins, and thehistamine content is low. The method disclosed by the invention is reasonable in process, advanced in technology and high in operability and can be applied to processing of the marine wastes.

Description

Technical field [0001] The present invention relates to a method for preparing peptides or proteins using fermentation or / and enzyme methods, in particular to a method for extracting nutrient solution from marine waste. Background technique [0002] We know that a large amount of seafood waste is produced in the fishery production process, which not only wastes resources but also pollutes the environment. If the targeted development of seafood waste can be designed, it will greatly save natural resources and bring good economic benefits. [0003] In the process of economic development of seafood waste, a major difficulty is that the freshness of seafood waste is often difficult to guarantee. Compared with the normal raw materials of seafood, seafood waste needs to be collected, aggregated and used for subsequent processing from different production facilities and different production steps. During this process, it may have a slight autolysis phenomenon, and cause the histamine co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L27/10A23L33/00
CPCA23L27/10A23L33/00A23V2002/00A23V2250/2042
Inventor 房文涛原永广
Owner 荣成市日鑫水产有限公司
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