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A nucleic acid sequence that can be used for molecular pest control and its application

A nucleic acid and sequence technology is applied in the application field of preventing and controlling Spodoptera litura, and can solve problems such as water pollution, pest generation, drug resistance and the like

Active Publication Date: 2020-12-18
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, chemical pesticides are mainly used for prevention and control. However, due to the large amount and long-term use of chemical pesticides, on the one hand, pests have developed drug resistance; on the other hand, they have brought about a series of problems such as environmental pollution, water pollution and human health.

Method used

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  • A nucleic acid sequence that can be used for molecular pest control and its application
  • A nucleic acid sequence that can be used for molecular pest control and its application
  • A nucleic acid sequence that can be used for molecular pest control and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] 1.4E specific primer design

[0052] Transcriptome data from Spodoptera litura After Parasitization by Microplitis bicoloratus.PLoS ONE 9( 10):e110967.doi:10.1371 / journal.pone.0110967), the 4E gene sequence was selected, the size of the 4E gene sequence (as shown in SEQ ID No.1) was 633bp, and a total of 210 amino acids were encoded (SEQ ID No.2). Specific primers were designed using the primer design software Primer Premier 5. Upstream primer L4440-4E-F (SEQ ID No. 3) and downstream primer L4440-4E-R (SEQ ID No. 4).

[0053] 2. Extraction of total RNA and cDNA synthesis of Spodoptera litura larvae

[0054] In this experiment, the laboratory protocol was used to extract total RNA from Spodoptera litura larvae. The specific steps are as follows:

[0055] 1) Take 10 3-day-old larvae, place them in EP tubes with a small amount of RNAisoTM Plus lysate, mash and grind them thoroughly, continue to add RNAisoTM Plus lysate until the total volume reaches 1ml, vortex and mix ...

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Abstract

The invention relates to a nucleic sequence applicable to molecular injurious insect prevention and application thereof. The nucleic sequence contains a nucleotide sequence of a nucleic sequence I, the nucleic sequence I is selected from the nucleotide sequence as shown by SEQ ID No.1 and one of the nucleotide sequences having a same function with the nucleotide sequence as shown by SEQ ID No.1 and capable of killing the spodoptera except for the spodoptera litura Fabricius. The nucleic sequence can be used for preventing and treating the spodoptera litura Fabricius.

Description

technical field [0001] The present application relates to a nucleic acid sequence that can be used for molecular control of pests, in particular to the application of the nucleic acid sequence in controlling pests of the genus Spodoptera. Background technique [0002] Spodoptera litura belongs to Lepidoptera Noctuidae, which is widely distributed all over the world and occurs in all parts of my country. It is a typical omnivorous insect. It is known that there are more than 290 kinds of plants in 99 families, including crops such as cotton, tobacco and vegetables. Because Spodoptera litura has strong reproductive ability and is easy to develop resistance to pesticides, in recent years, the damage to vegetables and flowers has become more and more serious, and the control difficulty has become more and more difficult. At present, chemical pesticides are mainly used for prevention and control. However, due to the extensive and long-term use of chemical pesticides, on the one ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113A01N37/46A01P7/04A61B5/107
CPCA01N37/46A61B5/1072A61B5/1079A61B2503/40A61B2503/42C12N15/113C12N2310/14
Inventor 罗开珺董树美李明崔继辉张玮张雪雯寇田超蔡秋宸张力丹何浩娟
Owner YUNNAN UNIV