Method for detection of thrombin based on enzyme and graphene oxide aptamer sensor
An aptamer sensor and thrombin technology, applied in the field of thrombin detection, can solve the problems of complex technology, long reaction time and low sensitivity, and achieve the effects of high-sensitivity identification, rapid detection and high sensitivity
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Embodiment 1
[0025] A method for detecting thrombin based on enzyme and graphene oxide aptamer sensor, comprising the following steps:
[0026] Step 1. Prepare 10 parts of thrombin standard solutions with different concentrations. The concentrations of the 10 parts of thrombin standard solutions are: 0mol / L, 5×10 -10 mol / L, 1×10 -9 mol / L, 2×10 -9 mol / L, 5×10 -9 mol / L, 1×10 -8 mol / L, 2×10 -8 mol / L, 4×10 -8 mol / L, 6×10 -8 mol / L, 1×10 -7 mol / L;
[0027] Step 2, prepare 10 parts of standard solutions to be tested, specifically:
[0028] Add the buffer solution to the thrombin aptamer DNA solution and add the complementary single-stranded DNA solution, put it in a water bath and raise the temperature to 93°C, turn off the switch of the water bath, and cool the water body in the water bath to room temperature to obtain double-stranded DNA Solution, wherein the buffer solution is a Tris-HCl buffer solution with a pH of 7.4 and a concentration of 0.075 mol / L, the volume ratio of the throm...
Embodiment 2
[0033] A method for detecting thrombin based on enzyme and graphene oxide aptamer sensor, comprising the following steps:
[0034] Step 1. Prepare 10 parts of thrombin standard solutions with different concentrations. The concentrations of the 10 parts of thrombin standard solutions are: 0mol / L, 5×10 -10 mol / L, 1×10 -9mol / L, 2×10 -9 mol / L, 5×10 -9 mol / L, 1×10 -8 mol / L, 2×10 -8 mol / L, 4×10 -8 mol / L, 6×10 -8 mol / L, 1×10 -7 mol / L;
[0035] Step 2, prepare 10 parts of standard solutions to be tested, specifically:
[0036] Add the buffer solution to the thrombin aptamer DNA solution and add the complementary single-stranded DNA solution, put it in a water bath and heat it up to 97°C, turn off the switch of the water bath, and cool the water body in the water bath to room temperature to obtain double-stranded DNA solution, wherein the buffer solution is a Tris-HCl buffer solution with a pH of 7.4 and a concentration of 0.075 mol / L, the volume ratio of the thrombin aptamer ...
Embodiment 3
[0041] A method for detecting thrombin based on enzyme and graphene oxide aptamer sensor, comprising the following steps:
[0042] Step 1. Prepare 10 parts of thrombin standard solutions with different concentrations. The concentrations of the 10 parts of thrombin standard solutions are: 0mol / L, 5×10 -10 mol / L, 1×10 -9 mol / L, 2×10 -9 mol / L, 5×10 -9 mol / L, 1×10 -8 mol / L, 2×10 -8 mol / L, 4×10 -8 mol / L, 6×10 -8 mol / L, 1×10 -7 mol / L;
[0043] Step 2, prepare 10 parts of standard solutions to be tested, specifically:
[0044] Add the buffer solution to the thrombin aptamer DNA solution and add the complementary single-stranded DNA solution, place it in a water bath and raise the temperature to 95°C, turn off the switch of the water bath, and cool the water body in the water bath to room temperature to obtain double-stranded DNA solution, wherein the buffer solution is a Tris-HCl buffer solution with a pH of 7.4 and a concentration of 0.075 mol / L, the volume ratio of the thr...
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