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Garland chrysanthemum bacteriostatic active monomer and application method thereof

A technology of antibacterial activity and chrysanthemum chrysanthemum, which is applied in chrysanthemum chrysanthemum antibacterial active monomer and its application field, can solve the problems of pathogenic bacteria resistance, carcinogenicity and teratogenicity of chemical fungicides, and achieve the effect of protecting growth and good inhibition

Inactive Publication Date: 2018-03-09
JIANGXI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the advancement of science and technology and the improvement of detection and analysis methods, some chemical fungicides that were considered safe in the past have been found to be carcinogenic or potentially carcinogenic, mutagenic, and teratogenic.
In addition, long-term use of chemical fungicides can easily cause pathogenic bacteria to develop resistance to them, reduce the control effect, and pollute the environment.

Method used

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  • Garland chrysanthemum bacteriostatic active monomer and application method thereof
  • Garland chrysanthemum bacteriostatic active monomer and application method thereof
  • Garland chrysanthemum bacteriostatic active monomer and application method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A kind of chrysanthemum chrysanthemum antibacterial active monomer is provided in embodiment 1, and the method is made up of the following steps:

[0030] S1: Chrysanthemum chrysanthemum stems and leaves pretreatment: Weigh 20Kg of fresh chrysanthemum chrysanthemum stems and leaves, dry them naturally in a blast dryer at 50°C for 12 hours, accurately weigh 2Kg of dried chrysanthemum chrysanthemums, and pulverize them in a Chinese herbal medicine grinder. Pass through a 40 mesh sieve;

[0031] S2: Extraction: add 50 L of 95% ethanol to ultrasonically assist extraction for 3 times, each time for 2 hours, combine the filtrate and concentrate under reduced pressure to obtain the extract;

[0032] S3: macroporous resin column chromatography: the extract was dissolved in 1.2L distilled water, filtered, and the supernatant was placed on a D101 macroporous resin column, followed by distilled water, 10% methanol, 30% methanol, 50% methanol, 70% methanol Methanol and 90% methano...

Embodiment 2

[0038] A kind of chrysanthemum chrysanthemum antibacterial activity monomer that embodiment 2 provides, the method is made up of the following steps:

[0039] S1: Chrysanthemum chrysanthemum stems and leaves pretreatment: Weigh 20Kg of fresh chrysanthemum chrysanthemum stems and leaves, dry them in a blast dryer at 45°C for 10 hours, accurately weigh 1.7Kg of dried chrysanthemum chrysanthemums, and pulverize them in a Chinese herbal medicine grinder , through a 40-mesh sieve;

[0040] S2: Extraction: add 48L of 95% ethanol to ultrasonically assist extraction twice, each time for 1 hour, combine the filtrate and concentrate under reduced pressure to obtain the extract;

[0041] S3: Macroporous resin column chromatography: the extract was dissolved in 1.1L distilled water, filtered, and the supernatant was placed on a D101 macroporous resin column, and distilled water, 10% methanol, 30% methanol, 50% methanol, 70% methanol Gradient elution with methanol and 90% methanol, 1.9L s...

Embodiment 3

[0047] A kind of chrysanthemum chrysanthemum antibacterial activity monomer that embodiment 3 provides, the method is made up of the following steps:

[0048] S1: Chrysanthemum chrysanthemum stems and leaves pretreatment: Weigh 20Kg of fresh chrysanthemum chrysanthemum stems and leaves, dry them in a blast dryer at 55°C for 14 hours, accurately weigh 2.3Kg of dried chrysanthemum chrysanthemums, and pulverize them in a Chinese herbal medicine grinder , through a 40-mesh sieve;

[0049] S2: Extraction: add 52L of 95% ethanol to ultrasonically assist extraction for 4 times, each time for 3 hours, combine the filtrate and concentrate under reduced pressure to obtain the extract;

[0050] S3: macroporous resin column chromatography: the extract was dissolved in 1.3L distilled water, filtered, and the supernatant was placed on a D101 macroporous resin column, followed by distilled water, 10% methanol, 30% methanol, 50% methanol, 70% methanol Methanol and 90% methanol gradient eluti...

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Abstract

The invention discloses a garland chrysanthemum bacteriostatic active monomer and an application method thereof and belongs to the technical field of separating and application methods of active materials. The garland chrysanthemum bacteriostatic active monomer is prepared through garland chrysanthemum stem and leaf pretreatment, liquid extraction, macroporous resin column chromatography, C18 medium-pressure chromatography, sephadex LH-20 column chromatography and semi-preparative liquid-phase separation. The garland chrysanthemum bacteriostatic active monomer is an extracted monomer with efficient bacteriostatic activity and can well inhibit watermelon fusarium oxysporum, thereby having a good and broad application prospect.

Description

technical field [0001] The invention relates to the technical field of separation and use of active substances, and more specifically, to a chrysanthemum chrysanthemum antibacterial active monomer and a use method thereof. Background technique [0002] Watermelon Fusarium wilt (Watermelon Fusariumwilt), also known as vine cutting disease, wilt disease, wilt disease, is called "watermelon cancer", caused by the fungus of the subphylum Fusarium oxysporum [Fusariumoxysporum f.sp.niveum (E.F.Smith ) Wollen] infection, is a devastating soil-borne disease that commonly occurs at home and abroad. Due to the rapid expansion of watermelon cultivation area, the area of ​​watermelon under heavy cropping has increased, and the occurrence of watermelon wilt has become more and more common and serious, which has brought huge economic losses and waste of resources to watermelon production. Fusarium wilt can cause watermelon production to drop by 30% to 40%, and seriously diseased plots ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/12A01P3/00
CPCA01N65/00A01N65/12
Inventor 范淑英张新龙李珊珊刘琳刘琼
Owner JIANGXI AGRICULTURAL UNIVERSITY