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Method for extracting glycolipids in spirulina with supercritical carbon dioxide

A spirulina and supercritical technology, applied in chemical instruments and methods, organic chemistry, sugar derivatives, etc., can solve the problems of low extraction rate of γ-linolenic acid, high extraction efficiency and high carbon dioxide consumption

Active Publication Date: 2020-12-08
GUOTOU BIO TECH INVESTMENT CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For example, Sajilata, M.G. et al. (J. Food Engineering 84, 321-326; 2008) reported the use of supercritical CO 2 Extraction is a method for obtaining gamma-linolenic acid from Spirulina, but the method has a low extraction rate of gamma-linolenic acid (compared with the Bligh&Dyer method, the extraction efficiency of gamma-linolenic acid is only 78%), and the consumption of carbon dioxide is large (200-2200ml min -1 ) and other shortcomings

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  • Method for extracting glycolipids in spirulina with supercritical carbon dioxide
  • Method for extracting glycolipids in spirulina with supercritical carbon dioxide
  • Method for extracting glycolipids in spirulina with supercritical carbon dioxide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1 The use of porous materials for supercritical CO 2 Extraction of Glycolipids from Spirulina and Effect of Extraction Rate of GLA-rich Glycolipids

[0063] ①Supercritical CO 2 Extractor boot preparation:

[0064] Open the gas bottle in advance to purge the whole system for a few minutes to remove the water vapor in the pipeline.

[0065] ② Weigh a certain amount of spirulina algae powder, use diatomite (60% water content, 20-40 mesh) of 0-1.0 times the quality of the raw material and mix it with the material for later use, and compare it with the sample without diatomite. Compared.

[0066] ③ According to spirulina raw material: ethanol = 1:3 (g / ml), add entrainer, mix evenly with the mixture in step S2, put it into the material bag, put it into the extraction kettle, install the sealing end cover of the extraction kettle and tighten the thread so that it does not There is a leak.

[0067] ④ Open the inlet and outlet valves of the extraction kettle, and us...

Embodiment 2

[0078] Example 2 The use of entrainer for supercritical CO 2 Extraction of total fatty acids in spirulina and the effect of GLA extraction rate

[0079] The specific operation steps are basically as described in Example 1. In the present embodiment, the ratio of entraining agent (food grade ethanol) and spirulina raw material is the consumption scope of 0-4 (volume mass ratio ml / g, be called for short " liquid material ratio "), other conditions are identical and as follows: diatomaceous earth / Spirulina raw material=0.5, water content of diatomaceous earth=60%, t=60min, T=40°C, P=6000psi, CO 2 The flow rate is 20ml·min -1 . The results are shown in Table 2 below.

[0080] Table 2

[0081]

[0082]

[0083] As shown in Table 2, the use of entrainer, especially ethanol, in the carbon dioxide supercritical extraction also helps to improve the extraction rate of glycolipids in Spirulina nutrients, especially the extraction rate of glycolipids MGDG and DGDG rich in GLA....

Embodiment 3

[0088] Embodiment 3 The influence of water content of spirulina raw material on extraction rate

[0089] Concrete operation steps are as embodiment 1. In the present embodiment, the scope of controlling the water content of the porous material diatomite is 0.11%-66.7%, other conditions are the same and as follows: diatomite / spirulina raw material=0.5, liquid-material ratio=3, t=60min, T =40°C, P=6000psi, CO 2 The flow rate is 20ml·min -1 . The results are shown in Table 4.

[0090] Table 4

[0091]

[0092] As shown in table 4, when making the porous material diatomite used in the present invention contain a certain amount of moisture, it will also help to improve the extraction rate of glycolipids in spirulina nutrients, especially the glycolipids MGDG and Extraction rate of DGDG. The data shows that when the water content of diatomaceous earth is 60%, the extraction rate is the highest. Compared with the common use of dried spirulina powder as raw material at prese...

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Abstract

The invention relates to a method for extracting glycolipid from spirulina. The method comprises the following steps: a spirulina raw material is collected; supercritical CO2 extraction is carried outon the spirulina raw material, wherein in the supercritical CO2 extraction process, an organic solvent is used as an entrainer, and a multipore material is added; an extract is collected, and then the extract is further extracted to obtain the glycolipid. The method disclosed by the invention is extremely high in extraction rate and extraction selectivity for spirulina extraction.

Description

technical field [0001] The application relates to the field of industrial application of microalgae, in particular to a method for obtaining glycolipids, especially glycolipids rich in γ-linolenic acid, from spirulina through supercritical carbon dioxide extraction. Background technique [0002] Spirulina (Spirulina), also known as "Arthrospira", belongs to Cyanophyceae, Oscillatoriaceae, and is a prokaryotic organism. Spirulina has attracted much attention because it is rich in various nutrients such as carotenoids, proteins, and lipids. Among the many nutrients contained in spirulina, glycolipids are one of the most concerned nutrients. Most of the lipids in spirulina are glycolipids, which have the functions of preventing cardiovascular diseases, lowering blood pressure, improving lipid metabolism, and lowering cholesterol. Moreover, monogalactose diacetylglyceride and digalactose diacetylglyceride in glycolipids contain γ-linolenic acid, and γ-linolenic acid also has s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H15/10C07H1/08
CPCC07H1/08C07H15/10Y02P20/54
Inventor 杨小红韩丹翔胡强
Owner GUOTOU BIO TECH INVESTMENT CO LTD