Augmented reality-based fixed-point de-kering method

An augmented reality, nuclear method technology, applied in the fields of botany equipment and methods, biochemical equipment and methods, genetic engineering, etc., can solve the problem of invisible position of the nucleus, and achieve the effect of improving the success rate of enucleation

Active Publication Date: 2021-06-01
NANKAI UNIV
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Problems solved by technology

It can solve the problem that the nucleus position is not visible in the blind aspiration enucleation operation, and improve the success rate of the enucleation operation

Method used

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  • Augmented reality-based fixed-point de-kering method
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  • Augmented reality-based fixed-point de-kering method

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Embodiment Construction

[0015] The cells used in this implementation example are domestic pig oocytes taken from a local slaughterhouse. The method of obtaining oocytes is as follows:

[0016] After the ovaries were removed from the slaughterhouse, they were transported to the laboratory within two hours in vacuum flasks filled with saline at 35° to 37°. Immediately wash twice with 37° sterile saline containing 100 IU / L penicillin and 50 mg / L streptomycin. The oocytes were extracted from the small sac with a diameter of 2 to 6 mm on the ovary, and the extracted cells were washed three times with TL-Hepes-PVA, and then matured in vitro (IVM) in an incubator at 39° with a carbon dioxide concentration of 5%42 Hour. After IVM, the cells were deovized with 0.1% hyaluronidase. Finally, the cells were washed three times with M199 to obtain the cells used. The MII stage oocytes of each test group to be tested were transferred into TCM199 containing 5 μg / ml Hoechst33342 and incubated for 15 minutes.

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Abstract

The invention discloses a fixed-point coring method based on augmented reality. The method is characterized in that the position model of the cell nucleus relative to the polar body is obtained by calculating the relative positional relationship between the polar body and the nucleus of the porcine oocyte just matured in vitro under a fluorescent microscope. Use augmented reality technology to mark the position of the nucleus on the oocyte to assist in the enucleation operation. During blind aspiration enucleation, adjust the position of the injection needle by marking the position of the nucleus. It solves the problem that the position of the cell nucleus is not visible during the blind aspiration enucleation process, and realizes the "visualization" effect of the cell nucleus. The success rate of enucleation and the consistency and repeatability of experimental operations are improved.

Description

technical field [0001] The invention relates to the technical field of somatic cell nuclear transfer. In particular it concerns the blind aspiration point enucleation method. Background technique [0002] Oocyte enucleation is a very important step in the process of nuclear transfer, and it is also the key to the success of nuclear transfer. During enucleation, the orientation of the nucleus determines whether enucleation is complete. At present, the methods of enucleation mainly include: Hoechst staining enucleation, chemical reagent enucleation, extrusion, blind aspiration, semi-egg method, end-stage enucleation, centrifugal enucleation, etc. Because the blind aspiration method does not rely on other chemical substances, it can reduce the damage that some chemical substances may cause to oocytes and its operation is simple and rapid, so it is widely used. The biggest problem with the blind aspiration method is that the position of the nuclei is not visible, and it canno...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/873
CPCC12N15/873
Inventor 赵新冯泽阳刘曜玮孙明竹崔茂盛
Owner NANKAI UNIV
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