Detection method of e.coli O157:H7 free from enzyme and fluorescence labeling

Abstract

The present invention provides a detection method for E.coli O157:H7 free of enzyme and fluorescent labeling, which comprises the following steps: hybridizing IS with an aptamer, the nucleotide sequence of said IS is as SEQ ID NO : 1, the nucleotide sequence of the adapter is shown in SEQ ID NO: 2; the resultant is placed in buffer, E.coli O157:H7 is added, and reacted for 30min at 37°C; GHP1 and GHP2, incubated at 37°C for 100 min; the nucleotide sequence of GHP1 is shown in SEQ ID NO: 3, and the nucleotide sequence of GHP2 is shown in SEQ ID NO: 4; NMM was added and incubated at 37°C 30min; the resultant was tested for fluorescence intensity.

Description

technical field [0001] The invention belongs to the technical field of bacterial detection, and in particular relates to an enzyme-free and fluorescent-label free detection method for Escherichia coli O157:H7. Background technique [0002] Escherichia coli O157:H7 is a common pathogen, and its detection is one of the key research topics in this field. Although some traditional detection methods can accurately detect Escherichia coli O157:H7, these methods usually take a long time, ranging from 2-3 days to more than 1 week. [0003] In recent years, techniques such as PCR, qPCR, ELASA and LAMP have been successively applied in the field of bacterial detection. Although the above method saves detection time, there are still many disadvantages, such as complicated operation, expensive equipment, and unsuitable storage of reagents. More importantly, the detection accuracy of these technologies is usually low and it is difficult to perform specific detection. It is not possible...

AI Technical Summary

Problems solved by technology

Although the above method saves detection time, there are still many disadvantages, such as complicated operation, expensive equipment, and unsuitable storage of reagents. More importantly, the detection accuracy of these technologies is usually low and it is difficult to perform specific detection. It is not possible to distinguish the different bacteria

[0004]In addition, most of the existing detection technologies require fluorescent labels, modification, or addition of enzymes, etc., which increase the cost of detection

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