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Method for qualitative and quantitative detection on glycoside structured surfactant

A quantitative detection method and surfactant technology, applied in the field of qualitative and quantitative detection of glycoside structure surfactants, can solve the problems of no response signal, inability to realize qualitative identification and quantitative detection, difficulty in separating glycoside structure surfactants, etc. , to achieve a wide range of effects

Inactive Publication Date: 2018-10-19
SHANGHAI WUXI BIOLOGIC TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved in the present invention is to provide a qualitative and quantitative detection method for glycoside structure surfactants, so as to solve the problem that it is difficult to separate glycoside structure surfactants with conventional detection methods, and there is no response signal, so that qualitative identification and quantitative detection cannot be realized. technical issues

Method used

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  • Method for qualitative and quantitative detection on glycoside structured surfactant
  • Method for qualitative and quantitative detection on glycoside structured surfactant
  • Method for qualitative and quantitative detection on glycoside structured surfactant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1 takes the sample of testing octyl-β-D-glucopyranoside (OGP) as an example:

[0048] 1. Mobile phase preparation:

[0049] 1.1. Mobile phase A: 0.1% v / v TFA (trifluoroacetic acid) in 10% v / vacetonitrile (acetonitrile);

[0050] Take 100mL of acetonitrile into a 1000mL solvent bottle, add 900mL of ultrapure water and 1mL of trifluoroacetic acid, and mix well;

[0051] 1.2. Mobile phase B: 0.1% v / v TFAin 90% v / vacetonitrile;

[0052] Take 900mL of acetonitrile into a 1000mL solvent bottle, add 100mL of ultrapure water and 1mL of trifluoroacetic acid, and mix well;

[0053] 1.3. Mobile phase C: pure water

[0054] Take 1L of pure water in a 1L solvent bottle;

[0055] 1.4. Mobile phase D: acetonitrile (column preservation solution);

[0056] Take 1L of acetonitrile in a 1L solvent bottle;

[0057] 1.5.Seal wash (cleaning the plunger rod seal): 10% IPA

[0058] Take 100mL isopropanol (IPA) and 900mL ultrapure water in a 1L solvent bottle and mix thoroughl...

Embodiment 2

[0097] Embodiment 2 takes the sample of testing decyl-β-D-glucopyranoside (DGP) as an example:

[0098] The experimental procedure of embodiment 2 is with embodiment 1, and experimental result is as follows:

[0099] 4. Test results

[0100] The reverse chromatogram of a sample containing decyl-β-D-glucopyranoside is shown in Figure 2-A As shown, decyl-β-D-glucopyranoside (injection volume 50 μL,) can be separated from the solvent peak with a retention time of 3.7 minutes.

[0101] 5. Glycosides are separated by a reverse chromatographic column and then enter a charged aerosol detector for detection

[0102] This embodiment uses a liquid phase-detector combination system, so when the glycosides are separated by the reverse chromatographic column, they will automatically enter the charged aerosol (CAD) detector through the pipeline.

[0103] The signal response was determined for the standard solution of decyl-β-D-glucopyranoside at different concentrations, and the logarit...

Embodiment 3

[0111] Embodiment 3 takes the sample of testing dodecyl-β-D-maltopyranoside (DMP) as an example:

[0112] The experimental procedure of embodiment 3 is with embodiment 1, and experimental result is as follows:

[0113] 4. Test results

[0114] The reverse chromatogram of a sample containing dodecyl-β-D-maltopyranoside is shown in Figure 3-A As shown, dodecyl-β-D-maltopyranoside (injection volume 50 μL,) can be separated from the solvent peak with a retention time of 5.3 minutes.

[0115] 5. Glycosides are separated by a reverse chromatographic column and then enter a charged aerosol detector for detection

[0116] This embodiment uses a liquid phase-detector combination system, so when the glycosides are separated by the reverse chromatographic column, they will automatically enter the charged aerosol (CAD) detector through the pipeline.

[0117] The signal response was measured for the standard solution of dodecyl-β-D-maltopyranoside at different concentrations, and the l...

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Abstract

The invention discloses a method for qualitative and quantitative detection on a glycoside structured surfactant. The method comprises the following steps: step 1: separating to-be-measured substancesin a glycoside structured surfactant sample by adopting a reverse-phase chromatography; step 2, after separation, enabling a glycoside compound to enter an electric fog detector for detection; step 3, performing quantitative measurement on the contents of the to-be-measured substances in the sample by adopting an external standard quantitative method. The method disclosed by the invention has thebenefits that the method combining the reverse-phase chromatography with the electric fog detector is established to perform qualitative and quantitative detection on glycosides with surfactant structures, so that the microgram per milliliter level of sample concentration can be accurately measured. The method is a high-throughput test method, and the to-be-measured glycosides in the sample are not required to be derived or modified. The invention provides the method which is wide in application range, accurate and effective for the qualitative and quantitative detection on the glycosides, thereby providing a strong support for dissolution, stability and characterization of membrane proteins.

Description

technical field [0001] The invention relates to a detection method for surfactants, in particular to a qualitative and quantitative detection method for surfactants with glycoside structures, especially the detection of glycoside structures by reverse chromatography (RP-HPLC) combined with a charged aerosol detector (CAD). The concentration and residual amount of amphoteric surfactant, octyl-β-D-glucopyranoside (OGP), etc. are qualitatively and quantitatively detected. Background technique [0002] Nonionic amphoteric surfactants play an important role in the dissolution, characterization and structural reorganization of membrane proteins (MPs). The micelles formed by this amphoteric surfactant will wrap the hydrophobic region of the membrane protein to form a protein-surfactant complex (protein–detergent complex, PDC). Although there are many surfactants to choose from, most of them are designed and produced for other industrial purposes, and very few are specially suitabl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/89G01N30/64
CPCG01N30/64G01N30/89
Inventor 孟博夏晓云袁燕秋汪波陆宇琛黄岗
Owner SHANGHAI WUXI BIOLOGIC TECH CO LTD
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