Microfluidic chip and application thereof

Abstract

The invention relates to the technical field of microfluidics and provides a microfluidic chip and an application thereof. The microfluidic chip comprises an upper substrate, a lower substrate, a first porous membrane and a second porous membrane, wherein a first liquid inlet, a second liquid inlet, a first liquid outlet, a second liquid outlet, a first chamber, a second chamber and a first microchannel are arranged on the upper substrate; the second liquid inlet, the first chamber, the second chamber and the second liquid outlet are sequentially communicated through the first microchannel; the lower substrate is provided with a third liquid inlet, a third liquid outlet, a third chamber, a fourth chamber and a second microchannel, and the third liquid inlet, the third chamber, the fourth chamber and the third liquid outlet are sequentially communicated through a second microchannel. According to the microfluidic chip, the four chambers are formed, upper fluid and lower fluid can be subjected to material exchange twice on porous membranes separating the four chambers, so that the blood purification process of glomerular filtration and tubular secretion and reabsorption of renal units can be simulated with high simulation degree.

Description

technical field [0001] The invention relates to a microfluidic chip and its application, belonging to the field of microfluidic technology. Background technique [0002] Microfluidics, also known as Lab-on-a-chip, is a science and technology for manipulating fluids in micron-scale space, which can reduce the basic functions of biological and chemical laboratories to one On a chip of several square centimeters, it is one of the most important cutting-edge technologies in the 21st century. It is considered to be the key technology to solve the key problems such as high R&D costs and long cycle of innovative drugs, cosmetics and health products, and to innovate the original technology system. We are facing great development opportunities and challenges. [0003] Organ on a chip is a subclass of microfluidic chip, which is a bionic technology that simulates organs by culturing one or more functional cells in a sheet of several square centimeters. The "organs" in the game are v...

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Problems solved by technology

[0006] Existing chips for simulating the material exchange membrane structure of the kidney system mainly include three-layer single-channel sandwich structures. For example, Ingber uses a three-layer single-channel sandwich structure to simulate the membrane structure formed by glomerular endothelial cells-podocytes , In another work, Ingber used this structure chip to simulate the single-layer channel chip of renal tubule structure. The membranous structure formed by the vascular endothelial cells of the glomerulus-podocytes, these structural units can only provide a material exchange area for the two fluids, and cannot effectively construct the structure of the glomeruli and renal tubules on the chip at the same time, thus The simulated nephron has a single structure and cannot effectively simulate the process of blood purification

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