Application of pink plumepoppy herb regulator capable of improving weaned pig immune performance

A technology for immune performance and weaned piglets, applied in the field of animal husbandry, can solve the problems of incomplete replacement of antibiotics, reduced feed utilization rate, residues, etc., and achieve the effects of improving disease resistance of piglets, improving feed utilization rate, and improving body immune performance

Inactive Publication Date: 2018-03-23
云南牧道生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The long-term use of antibiotics in large quantities leads to bacterial drug resistance and drug residues, which has aroused great concern in the society. However, the adverse effects of prohibiting the use of antibiotics on the pig breeding industry have gradually emerged. reduce
At present, there is no additive that can effectively improve the performance of pigs and improve feed utilization like antibiotics. If antibiotics are not used in pig feed, it will lead to increased feed consumption and decreased growth rate of pigs. Therefore, stop using antibiotics Feed additives must have new antibiotic substitut

Method used

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  • Application of pink plumepoppy herb regulator capable of improving weaned pig immune performance
  • Application of pink plumepoppy herb regulator capable of improving weaned pig immune performance
  • Application of pink plumepoppy herb regulator capable of improving weaned pig immune performance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A kind of application of the pulse drop regulator that improves weaned piglet immunity performance, comprises the following steps:

[0028] A1. Antibacterial test

[0029] Escherichia coli, Salmonella, Staphylococcus aureus, and Bacillus subtilis were selected as test objects to determine the antibacterial efficacy and minimum inhibitory concentration of different concentrations of Boluohui extract. The minimum inhibitory concentrations of coccus and Bacillus subtilis are 1280μg / ml, 1280μg / ml, 2560μg / ml, 2560μg / ml respectively;

[0030] A2. Animal feeding experiment

[0031] 60 PIC five-element hybrid piglets were selected as experimental animals, weaned at the age of 35 days, and the experiment adopted a 1×4+1 randomized design, and were randomly divided into 5 treatment groups, with 4 replicates in each group, 3 piglets in each replicate, and piglets in each treatment The test diets added with 0mg / kg, 2.5mg / kg, 5.0mg / kg, and 7.5mg / kg Boluohui extract were fed respec...

Embodiment 2

[0036] On the basis of Example 1, in the step A1, the preparation steps of the Boluohui extract are as follows: take the dried aerial part of Boluohui, grind it to a thickness of 40-50 mesh, and then add 80% ethanol with a pH of 5 , filtered after reflux for 2 hours, combined the filtrates and concentrated until the ethanol was recovered completely, added an equal volume of water, stood still, a precipitate was obtained, and the supernatant was removed.

[0037] The obtained Boluohui extract has high purity and simple operation.

Embodiment 3

[0039]On the basis of Example 1-2, in step A1, the step of antibacterial efficacy determination is: respectively pipette 0.1ml of the bacterium liquid of Escherichia coli, Salmonella, Staphylococcus aureus, Bacillus subtilis in the logarithmic growth phase , spread evenly on the surface of ordinary broth culture medium, take a sterilized filter paper piece with a diameter of 6mm and immerse it in 1.5mg / ml, 3.0mg / ml, 4.5mg / ml Boluohui extract solution for 40min, take it out, wait for it to dry and put it into the inoculation In the plate, place the plate in a 37°C incubator for 24 hours and observe it. Take 3 pieces of paper for each type of bacteria and record the average value of the diameter of the inhibition zone. The piglets in the non-supplementation regulator group significantly increased the serum IgG content by 5 to 6 times, and the serum IgG content in piglets in the Posterior regulator group was 3 to 4 times higher than that in the antibiotic-positive group. Boluohui...

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PUM

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Abstract

The invention belongs to the technical field of livestock breeding and specifically relates to application of a pink plumepoppy herb regulator capable of improving weaned pig immune performance. The application comprises the following steps: A1, a bacteriostatic test; A2, an animal feeding test; A3, a cell test. The pink plumepoppy herb regulator has an obvious bacteriostatic effect on pathogenicmicroorganisms, the pink plumepoppy herb regulator has an bacteriostatic effect on gram positive bacteria or gram negative bacteria, and an antibacterial spectrum testing result of the pink plumepoppyherb regulator to the pathogenic microorganisms is staphylococcus aureus larger than bacillus subtilis larger than escherichia coli larger than salmonella. When being applied, the pink plumepoppy herb regulator has the bacteriostatic effect on all weaned pig enteropathogenic microorganisms like the escherichia coli, the salmonella and the staphylococcus aureus and especially has the most remarkable bacteriostatic effect on the staphylococcus aureus.

Description

technical field [0001] The invention belongs to the technical field of animal husbandry, and in particular relates to the application of a hysteresis regulator for improving the immune performance of weaned piglets. Background technique [0002] The long-term use of antibiotics in large quantities leads to bacterial drug resistance and drug residues, which has aroused great concern in the society. However, the adverse effects of prohibiting the use of antibiotics on the pig breeding industry have gradually emerged. reduce. At present, there is no additive that can effectively improve the performance of pigs and improve feed utilization like antibiotics. If antibiotics are not used in pig feed, it will lead to increased feed consumption and decreased growth rate of pigs. Therefore, stop using antibiotics Feed additives must have new antibiotic substitutes to ensure the health and production efficiency of pigs. Currently commonly used enzyme preparations, trace element additi...

Claims

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Application Information

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IPC IPC(8): C12Q1/6851C12Q1/18C12Q1/14C12Q1/10
CPCC12Q1/10C12Q1/14C12Q1/18C12Q1/6851G01N2333/245G01N2333/255G01N2333/31G01N2333/32C12Q2561/113C12Q2563/107
Inventor 计乔平郭荣富张春勇安清聪李锐王红琴陈启红王小强王丽郭永兵
Owner 云南牧道生物技术有限公司
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