Application of pink plumepoppy herb regulator capable of improving weaned pig immune performance
A technology for immune performance and weaned piglets, applied in the field of animal husbandry, can solve the problems of incomplete replacement of antibiotics, reduced feed utilization rate, residues, etc., and achieve the effects of improving disease resistance of piglets, improving feed utilization rate, and improving body immune performance
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Embodiment 1
[0027] A kind of application of the pulse drop regulator that improves weaned piglet immunity performance, comprises the following steps:
[0028] A1. Antibacterial test
[0029] Escherichia coli, Salmonella, Staphylococcus aureus, and Bacillus subtilis were selected as test objects to determine the antibacterial efficacy and minimum inhibitory concentration of different concentrations of Boluohui extract. The minimum inhibitory concentrations of coccus and Bacillus subtilis are 1280μg / ml, 1280μg / ml, 2560μg / ml, 2560μg / ml respectively;
[0030] A2. Animal feeding experiment
[0031] 60 PIC five-element hybrid piglets were selected as experimental animals, weaned at the age of 35 days, and the experiment adopted a 1×4+1 randomized design, and were randomly divided into 5 treatment groups, with 4 replicates in each group, 3 piglets in each replicate, and piglets in each treatment The test diets added with 0mg / kg, 2.5mg / kg, 5.0mg / kg, and 7.5mg / kg Boluohui extract were fed respec...
Embodiment 2
[0036] On the basis of Example 1, in the step A1, the preparation steps of the Boluohui extract are as follows: take the dried aerial part of Boluohui, grind it to a thickness of 40-50 mesh, and then add 80% ethanol with a pH of 5 , filtered after reflux for 2 hours, combined the filtrates and concentrated until the ethanol was recovered completely, added an equal volume of water, stood still, a precipitate was obtained, and the supernatant was removed.
[0037] The obtained Boluohui extract has high purity and simple operation.
Embodiment 3
[0039]On the basis of Example 1-2, in step A1, the step of antibacterial efficacy determination is: respectively pipette 0.1ml of the bacterium liquid of Escherichia coli, Salmonella, Staphylococcus aureus, Bacillus subtilis in the logarithmic growth phase , spread evenly on the surface of ordinary broth culture medium, take a sterilized filter paper piece with a diameter of 6mm and immerse it in 1.5mg / ml, 3.0mg / ml, 4.5mg / ml Boluohui extract solution for 40min, take it out, wait for it to dry and put it into the inoculation In the plate, place the plate in a 37°C incubator for 24 hours and observe it. Take 3 pieces of paper for each type of bacteria and record the average value of the diameter of the inhibition zone. The piglets in the non-supplementation regulator group significantly increased the serum IgG content by 5 to 6 times, and the serum IgG content in piglets in the Posterior regulator group was 3 to 4 times higher than that in the antibiotic-positive group. Boluohui...
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