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Systems and methods for image processing in optical microscopy

A technology of optical microscopy and microscopy, applied in the direction of image data processing, microscopy, capturing objects visible under the microscope, etc., can solve the problems of failure to improve asymmetry, elongation, etc.

Active Publication Date: 2022-04-05
LEICA MICROSYSTEMS CMS GMBH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Applying conventional deconvolution to these images improved resolution and contrast, but failed to improve the asymmetry (stretching) caused by the asymmetrical optics

Method used

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  • Systems and methods for image processing in optical microscopy
  • Systems and methods for image processing in optical microscopy
  • Systems and methods for image processing in optical microscopy

Examples

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Embodiment Construction

[0024] Example embodiments are described with reference to the figures, wherein like components are identified by like numerals. The description of these embodiments is exemplary, and is not intended to limit the scope of the present disclosure.

[0025]An example embodiment is a method for processing images in optical microscopy. The method involves acquiring raw images using a microscope such as a line-scanning confocal microscope. Raw images obtained from such microscopes can have asymmetric resolution. The method includes asymmetrically deconvolving at least a portion of the original image using a different point spread function in the X direction than in the Y direction to generate an asymmetrically deconvolved image. The method also includes displaying the asymmetrically deconvolved image on a display device.

[0026] According to some example embodiments, the original image may have an elliptical point spread function with confocality in the Y direction and wide fiel...

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Abstract

Example embodiments relate to systems and methods for processing images in optical microscopy, such as for a CMOS camera used as an optical detector in a line confocal fluorescence imager. The method includes: collecting an original image using a microscope; and asymmetrically deconvolving at least a portion of the original image using a point spread function that is different in the X direction than in the Y direction to generate an asymmetrically deconvolved image. Image. The method also includes compressing CMOS camera noise when the image is a monochromatic fluorescence image. Also provided is a system for processing images in optical microscopy and an image processing system for processing monochrome images from a CMOS camera-based line scan confocal fluorescence microscope.

Description

technical field [0001] Example embodiments disclosed herein generally relate to line scan imaging systems. More particularly, the disclosed example embodiments relate to systems and methods for processing images in a line confocal fluorescence microscope. Background technique [0002] Typically, when studying tiny regions of interest on a sample, researchers use a fluorescence microscope to view the sample. Microscopes can be conventional widefield, structured light or confocal microscopes. The optical configuration of such a microscope typically includes a light source, illumination optics, objective lens, sample holder, imaging optics and detectors. Light emitted from the light source illuminates a region of interest on the sample after propagating through the illumination optics and objective lens. The microscope objective forms a magnified image of the object that can be viewed through the eyepiece, or in the case of a digital microscope, the magnified image is captur...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G02B21/00G02B21/16G02B21/36G06V10/30
CPCG06T2207/10056G06T2207/30024G02B21/365G02B21/008G02B21/0076G06V20/693G06V20/695G06V10/30H04N23/80G06T5/73
Inventor P.C.戈德温
Owner LEICA MICROSYSTEMS CMS GMBH